[Primary exploration on immune associated genome of patients with Pi-Qi deficiency syndrome].
[Primary exploration on immune associated genome of patients with Pi-Qi deficiency syndrome].
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发表时间：
2005-04
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影响因子：
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通讯作者：
Yun-Jian Luo;Zong-Chang Xiu;Sui-Ping Huang
中科院分区：
文献类型：
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作者：
Yun-Jian Luo;Zong-Chang Xiu;Sui-Ping Huang
目的研究脾气虚证患者免疫功能的异常变化，并应用基因芯片技术探讨脾气虚证患者免疫功能异常的基因组学机制。方法提取健康人、慢性胃炎和溃疡性结肠炎患者外周血白色细胞（WBC）的总RNA和mRNA，分别用Cy3和Cy5标记，经显微放大制备交叉探针。然后将等量的两种标记探针混合后与cDNA芯片杂交，用扫描仪扫描芯片的荧光信号。比较正常人和患者外周血白细胞免疫相关基因组表达水平的差异。结果胃炎和结肠炎患者WBC中CD9、CD164、PF4和RARB基因表达下调，IGKC、DEFA 1和GNLY基因表达上调。结论脾气虚证的发生有其免疫相关的基因组学基础，脾气虚证患者存在免疫功能紊乱。
OBJECTIVE To investigate the abnormal change of immune function in patients with Pi-Qi deficiency Syndrome, and to explore the genomic mechanism of its genesis by cDNA chip techniques. METHODS The cross probe was made by extracting and microamplifying the total RNA and mRNA of peripheral white blood cells (WBC) in healthy subjects and patients with chronic gastritis and ulcerative colitis, which were labeled by Cy3 and Cy5 respectively. Then equal quantity of the two labeled probes were mixed and hybridized with cDNA chip, fluorescent signal of the chips were scanned with scanner. Data obtained were analyzed for comparing the difference of the expressive levels of immune associated genome in peripheral WBC in healthy subjects with those in patients. RESULTS Expressions of CD9, CD164, PF4 and RARB gene in WBC of patients, both gastritis and colitis, were down-regulated while those of IGKC, DEFA1 and GNLY were up-regulated. CONCLUSION The genesis of Pi-Qi deficiency syndrome has its immune associated genomic basis, and the immune functions are disordered in patients with that syndrome.