PREPARATION AND EVALUATION OF AN ONLINE PRECONCENTRATOR FOR CAPILLARY ELECTROPHORESIS
PREPARATION AND EVALUATION OF AN ONLINE PRECONCENTRATOR FOR CAPILLARY ELECTROPHORESIS
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DOI：
10.1002/mcs.1220050405
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发表时间：
1993-07-01
期刊：
影响因子：
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通讯作者：
JORGENSON, JW
中科院分区：
文献类型：
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作者：
HOYT, AM;BEALE, SC;JORGENSON, JW
描述了用于毛细管电泳 (CE) 的在线预浓缩器的制备和性能。将硼硅酸盐玻璃微球干装到熔融石英柱末端，并用为此应用开发的微电弧烧结到位。将反相LC填充材料浆料填充到该玻璃料上，长度为1-3mm。预浓缩器后面的额外硼硅酸盐玻璃料用于将填充床固定到位。对填充床预浓缩器增强紫外和荧光检测限的性能进行了评估。描述了微电弧装置的构造、包装程序和实际应用。
The preparation and performance of an on-line preconcentrator for capillary electrophoresis (CE) is described. Borosilicate glass microspheres were dry-packed into the fused silica column terminus and sintered in place with a micro electric arc developed for this application. Reversed-phase LC packing material was slurry-packed onto this frit, in lengths of 1-3 mm. An additional frit of borosilicate beads behind the preconcentrator was used to fix the packed bed in place. The performance of the packed bed preconcentrator for the enhancement of UV and fluorescence detection limits was evaluated. Construction of the micro electric arc device, the packing procedures, and practical applications are described.