Morphological and electrophysiological properties of atypically oriented layer 2 pyramidal cells of the juvenile rat neocortex.
Morphological and electrophysiological properties of atypically oriented layer 2 pyramidal cells of the juvenile rat neocortex.
复制标题
幼年大鼠新皮质非典型定向第 2 层锥体细胞的形态学和电生理学特性。
DOI：
10.1016/s0306-4522(00)00430-9
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发表时间：
2000
期刊：
影响因子：
3.3
通讯作者：
Spain,WJ
中科院分区：
文献类型：
--
作者：
vanBrederode,JF;Foehring,RC;Spain,WJ
我们使用全细胞膜片钳记录结合细胞内染料填充，以研究形态学和电生理特性的定向锥体细胞位于1/2层的边缘的幼年大鼠新皮层。顶端树突的取向从倾斜（与垂直成>20°）到真正水平（与垂直成90°）变化。顶端树突的长度为150 ~ 400μm。最长水平锥体的树突树（包括基底树突）的总水平域超过500μm，但我们也发现了水平树突域小于300μm的短水平细胞。此外，在第1/2层中，定向锥体具有长的水平轴突侧支。在电生理学上，神经定向的锥体细胞具有与在该年龄大鼠的浅层中描述的规则定向的锥体类似的内在膜特性。重复放电的细胞都是有规律的尖峰信号。此外，我们在这个样本中发现了一个神经元亚组（20%），它们在电流脉冲开始时无法发出超过几个尖峰。我们认为，独特的方向和大小的树突状树和长度和安排的本地轴突侧支，使神经定向金字塔层2非常适合执行水平整合的突触输入的新皮层。
We used whole-cell patch clamp recordings combined with intracellular dye-filling to examine the morphological and electrophysiological properties of atypically oriented pyramidal cells located at the layer 1/2 border of the juvenile rat neocortex. Orientation of the apical dendrite varied from oblique (>20° from vertical) to truly horizontal (90° from vertical). The length of the apical dendrite ranged from 150 to 400μm. The total horizontal domain of the dendritic tree (including basal dendrites) of the longest horizontal pyramids exceeded 500μm, but we also found short horizontal cells with horizontal dendritic domains of less than 300μm. In addition, atypically oriented pyramids had long horizontal axon collaterals in layer 1/2. Electrophysiologically, atypically oriented pyramidal cells had intrinsic membrane properties similar to regularly oriented pyramids that have been described in the superficial layers at this age in the rat. Cells that fired repetitively were all regular spiking. In addition, we identified a subgroup of neurons (20%) in this sample, which were unable to fire more than a few spikes at the beginning of the current pulse. We suggest that the unique orientation and size of their dendritic trees and the length and arrangement of their local axons collaterals make atypically oriented pyramids in layer 2 ideally suited to perform horizontal integration of synaptic inputs in the neocortex.