Imaging cervical cytology with scanning near-field optical microscopy (SNOM) coupled with an IR-FEL.
Imaging cervical cytology with scanning near-field optical microscopy (SNOM) coupled with an IR-FEL.
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DOI：
10.1038/srep29494
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发表时间：
2016-07-12
影响因子：
4.6
通讯作者：
Martin FL
中科院分区：
文献类型：
--
作者：
Halliwell DE;Morais CL;Lima KM;Trevisan J;Siggel-King MR;Craig T;Ingham J;Martin DS;Heys KA;Kyrgiou M;Mitra A;Paraskevaidis E;Theophilou G;Martin-Hirsch PL;Cricenti A;Luce M;Weightman P;Martin FL
宫颈癌仍然是妇女发病和死亡的主要原因，特别是在发展中国家。蛋白质、脂质和核酸的合成增加是癌细胞快速增殖的先决条件。我们表明，扫描近场光学显微镜，结合红外自由电子激光（SNOM-IR-FEL），能够区分正常和鳞状低度和高度核异常，正常和混合鳞状/腺浸润前和腺癌宫颈病变，在指定的波长与DNA，酰胺I/II和脂质。这些发现证明了SNOM-IR-FEL技术在以低于衍射极限（≥0.2 μm）的空间分辨率获得与宫颈细胞异常检测和癌症诊断相关的化学信息方面的前景。我们将这些结果与衰减全反射傅里叶变换红外（ATR-FTIR）光谱分析进行比较;尽管后一种方法已被证明可以检测传统细胞学遗漏的潜在宫颈糜烂，但由于光学衍射限制，其空间分辨率仅为3 μm至30 μm。
Cervical cancer remains a major cause of morbidity and mortality among women, especially in the developing world. Increased synthesis of proteins, lipids and nucleic acids is a pre-condition for the rapid proliferation of cancer cells. We show that scanning near-field optical microscopy, in combination with an infrared free electron laser (SNOM-IR-FEL), is able to distinguish between normal and squamous low-grade and high-grade dyskaryosis, and between normal and mixed squamous/glandular pre-invasive and adenocarcinoma cervical lesions, at designated wavelengths associated with DNA, Amide I/II and lipids. These findings evidence the promise of the SNOM-IR-FEL technique in obtaining chemical information relevant to the detection of cervical cell abnormalities and cancer diagnosis at spatial resolutions below the diffraction limit (≥0.2 μm). We compare these results with analyses following attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy; although this latter approach has been demonstrated to detect underlying cervical atypia missed by conventional cytology, it is limited by a spatial resolution of ~3 μm to 30 μm due to the optical diffraction limit.