Spacer-length dependence of programmed -1 or -2 ribosomal frameshifting on a U6A heptamer supports a role for messenger RNA (mRNA) tension in frameshifting.
Spacer-length dependence of programmed -1 or -2 ribosomal frameshifting on a U6A heptamer supports a role for messenger RNA (mRNA) tension in frameshifting.
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DOI：
10.1093/nar/gks629
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发表时间：
2012-09-01
影响因子：
14.9
通讯作者：
Brierley I
中科院分区：
文献类型：
--
作者：
Lin Z;Gilbert RJ;Brierley I
Programmed −1 ribosomal frameshifting is employed in the expression of a number of viral and cellular genes. In this process, the ribosome slips backwards by a single nucleotide and continues translation of an overlapping reading frame, generating a fusion protein. Frameshifting signals comprise a heptanucleotide slippery sequence, where the ribosome changes frame, and a stimulatory RNA structure, a stem–loop or RNA pseudoknot. Antisense oligonucleotides annealed appropriately 3′ of a slippery sequence have also shown activity in frameshifting, at least in vitro. Here we examined frameshifting at the U6A slippery sequence of the HIV gag/pol signal and found high levels of both −1 and −2 frameshifting with stem–loop, pseudoknot or antisense oligonucleotide stimulators. By examining −1 and −2 frameshifting outcomes on mRNAs with varying slippery sequence-stimulatory RNA spacing distances, we found that −2 frameshifting was optimal at a spacer length 1–2 nucleotides shorter than that optimal for −1 frameshifting with all stimulatory RNAs tested. We propose that the shorter spacer increases the tension on the mRNA such that when the tRNA detaches, it more readily enters the −2 frame on the U6A heptamer. We propose that mRNA tension is central to frameshifting, whether promoted by stem–loop, pseudoknot or antisense oligonucleotide stimulator.
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影响因子：
5.6
作者：
Brierley I;Rolley NJ;Jenner AJ;Inglis SC
通讯作者：
Inglis SC
影响因子：
64.5
作者：
Jacks T;Madhani HD;Masiarz FR;Varmus HE
通讯作者：
Varmus HE
DOI：
10.1073/pnas.0905046106
发表时间：
2009-08-04
影响因子：
11.1
作者：
Chen, Gang;Chang, Kung-Yao;Tinoco, Ignacio, Jr.
通讯作者：
Tinoco, Ignacio, Jr.
影响因子：
5.4
作者：
Knops, Elena;Brakier-Gingras, Lea;Verheyen, Jens
通讯作者：
Verheyen, Jens
DOI：
10.1099/vir.0.042499-0
发表时间：
2012-07
期刊：
The Journal of general virology
影响因子：
--
作者：
Firth AE;Brierley I
通讯作者：
Brierley I