THE ISOLATION OF ABSCISIC-ACID (ABA) DEFICIENT MUTANTS BY SELECTION OF INDUCED REVERTANTS IN NON-GERMINATING GIBBERELLIN SENSITIVE LINES OF ARABIDOPSIS-THALIANA (L) HEYNH
THE ISOLATION OF ABSCISIC-ACID (ABA) DEFICIENT MUTANTS BY SELECTION OF INDUCED REVERTANTS IN NON-GERMINATING GIBBERELLIN SENSITIVE LINES OF ARABIDOPSIS-THALIANA (L) HEYNH
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DOI：
10.1007/bf00272861
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发表时间：
1982-01-01
影响因子：
5.4
通讯作者：
KARSSEN, CM
中科院分区：
文献类型：
--
作者：
KOORNNEEF, M;JORNA, ML;KARSSEN, CM
通过选择拟南芥中 ga-1 基因座经诱变剂处理的非发芽赤霉素响应矮化突变体的后代中的发芽种子，可以分离发芽系（回复突变体）。大约一半的回复体是纯合隐性基因 (aba)，该基因可能调节脱落酸 (ABA) 的存在。关于该基因的功能的论证仅从该基因座的纯合隐性品系获得，该品系通过从回复突变体×的F2后代中选择而获得。野生型杂交。这些品系的特征是种子休眠减少、枯萎症状、蒸腾作用增加以及发育和成熟的种子和叶子中ABA含量降低。
By selecting for germinating seeds in the progeny of mutagen-treated non-germinating gibberellin responsive dwarf mutants of the ga-1 locus in A. thaliana, germinating lines (revertants) could be isolated. About half of the revertants were homozygous recessive for a gene (aba), which probably regulates the presence of abscisic acid (ABA). Arguments for the function of this gene were obtained from lines homozygous recessive for this locus only, obtained by selection from the F2 progeny of revertant .times. wild-type crosses. These lines are characterized by a reduced seed dormancy, symptoms of withering, increased transpiration and a lowered ABA content in developing and ripe seeds and leaves.