Rapid assay for detection of methicillin-resistant Staphylococcus aureus using multiplex PCR
Rapid assay for detection of methicillin-resistant Staphylococcus aureus using multiplex PCR
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DOI：
10.1006/mcpr.1996.0066
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发表时间：
1996-12-01
影响因子：
3.3
通讯作者：
Kur, J
中科院分区：
文献类型：
--
作者：
Barski, P;Piechowicz, L;Kur, J
通过多重聚合酶链式反应 (MPCR) 在金黄色葡萄球菌的临床分离株中检查了 mecA 基因的存在或缺失，mecA 基因是对所有 β-内酰胺抗生素耐药性的决定因素。使用两对引物，产生两种特定产物；一个基于 nuc 的 280 bp PCR 片段（编码特定金黄色葡萄球菌核酸酶的 nuc 基因的扩增产物）和一个基于 mecA 的 533 bp PCR 片段（mecA 基因的扩增产物）。 MPCR 系统旨在作为常规分析纳入临床诊断实验室的工作流程。 (C) 1996 学术出版社有限公司
The presence or absence of the mecA gene, the determinant of resistance to all beta-lactam antibiotics, was examined in clinical isolates of Staphylococcus aureus by multiplex polymerase chain reaction (MPCR). Two pairs of primers were used, which yielded two specific products; a 280-bp nuc-based PCR fragment (amplification product of the nuc gene encoding specific Staphylococcus aureus nuclease) and a 533-bp mecA-based PCR fragment (amplification product of the mecA gene). The MPCR system was designed to be incorporated into the work flow in clinical diagnostic laboratories as a routine analysis. (C) 1996 Academic Press Limited