ISOMYOSIN DISTRIBUTION IN NORMAL AND PRESSURE-OVERLOADED RAT VENTRICULAR MYOCARDIUM - AN IMMUNOHISTOCHEMICAL STUDY
ISOMYOSIN DISTRIBUTION IN NORMAL AND PRESSURE-OVERLOADED RAT VENTRICULAR MYOCARDIUM - AN IMMUNOHISTOCHEMICAL STUDY
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DOI：
10.1161/01.res.49.4.1003
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发表时间：
1981-01-01
影响因子：
20.1
通讯作者：
SCHIAFFINO, S
中科院分区：
文献类型：
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作者：
GORZA, L;PAULETTO, P;SCHIAFFINO, S
用亲和纯化的抗体与豚鼠比目鱼肌和心室肌球蛋白重链反应，分析特异性异肌球蛋白在正常大鼠和肾性高血压大鼠心肌中的分布。心脏组织切片2抗肌球蛋白免疫荧光染色显示，心室肌纤维之间的反应性有显著差异，左室比右室多，心内膜下比心外膜下多。在发育过程中，心肌的反应发生了变化：新生大鼠的心肌纤维全部染色，而1月龄大鼠的心肌肌纤维大部分为无反应。反应纤维的数量在随后的阶段增加，导致成年动物出现混合模式。在高血压大鼠2个月时，正常的混合型反应性转变为均匀阳性。手术后。在接受检查的23只高血压动物中，有20只观察到了这种完全的转变。这2种抗肌球蛋白与一种特殊类型的心肌肌球蛋白重链发生交叉反应，这种重链在不同的肌细胞中分布不同，在发育过程中和全身性高血压引起的心肌肥厚过程中其相对浓度也会发生变化。显然，压力负荷的差异可能是高血压动物异肌球蛋白分布的区域性差异和异肌球蛋白变化的原因。
Affinity-purified antibodies reacting with guinea pig soleus muscle and ventricular myosin heavy chains were used to analyze the distribution of specific isomyosin in the ventricular myocardium of normal and renal hypertensive rats. Immunofluorescent staining of cardiac tissue sections with the 2 antimyosins revealed striking variations in reactivity among ventricular muscle fibers, reactive fibers being more numerous in the left compared to the right ventricle and in subendocardial compared to subepicardial layers. The response of the ventricular myocardium changed during development: all fibers were stained in the newborn rat, while most fibers were unreactive in 1-mo-old animals. The number of reactive fibers increased in subsequent stages leading to a mixed pattern in adult animals. The normal mixed pattern of reactivity was transformed into a uniformly positive pattern in hypertensive rats 2 mo. after surgery. This complete transformation was observed in 20 of 23 hypertensive animals examined. The 2 antimyosins evidently cross-react with a particular type of ventricular myosin heavy chain, whose distribution varies in different muscle cells and whose relative concentration changes during development and during cardiac hypertrophy induced by systemic hypertension. Apparently, differences in pressure load may be responsible for both regional variations in isomyosin distribution and for isomyosin changes in hypertensive animals.