Piscinibacter caeni sp nov., isolated from activated sludge
Piscinibacter caeni sp nov., isolated from activated sludge
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从活性污泥中分离出卡氏鱼新属 (Piscinibacter caeni sp nov.)
DOI：
10.1099/ijsem.0.002891
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发表时间：
2018
影响因子：
2.8
通讯作者：
Chen Jian-Meng
中科院分区：
文献类型：
--
作者：
Chen Dong-Zhi;Yu Ning-Ning;Chu Qi-Ying;Chen Jing;Ye Jie-Xu;Cheng Zhuo-Wei;Zhang Shi-Han;Chen Jian-Meng
从浙江某制药厂的活性污泥中分离到一株黄色细菌MQ-18 T。该菌株的特点是通过多相分类法。16 S rRNA基因序列分析表明，菌株MQ-18 T与Piscinibacter defluviiSH-1 T（99.7%）和Piscinibacter aquaticusIMCC 1728 T（98.4%）的同源性较高，属于Piscinibacter属。 该菌株与SH-1 T和IMCC 1728 T的DNA-DNA同源性分别为35.4%和33.3%。 MQ-18 T细胞为革兰氏阴性、需氧、能运动、杆状、不产芽孢。该菌株在25-37 °C（最佳：30 °C）、0- 3.0%（w/v）NaCl（最佳，0%NaCl）存在下和pH 5.0-8.0（pH 7.0）下表现出生长。 主要脂肪酸为C12：0（5.5%）、C16：0（33.7%）、总特征3（C16：1ω 7 c和/或C16：1ω 6 c; 38.5%）和总特征4（anteiso-C17：1B和/或iso C17：1 I; 11.6%）。 醌类主要为泛醌-8，多胺主要为尸胺和腐胺。主要极性脂质谱由二磷脂酰甘油、磷脂酰甘油和磷脂酰乙醇胺组成。DNA G+C含量为70.1mol%。MQ-18 T菌株的系统发育、表型和生理特性被认为是Piscinibacter属的一个新种，命名为Piscinibacter caenisp。十一是建议。模式菌株为MQ-18 T（CCTCC AB 2017223 T =JCM 32138 T）。
A yellowish-pigmented bacterial strain, designated as MQ-18T, was isolated from a sample of activated sludge collected from a pharmaceutical factory in Zhejiang, China. The strain was characterized through a polyphasic taxonomy approach. 16S rRNA gene sequence analysis demonstrated that strain MQ-18Tshowed high similarities toPiscinibacter defluviiSH-1T(99.7 %) andPiscinibacter aquaticusIMCC1728T(98.4 %), thereby suggesting that it belongs to the genusPiscinibacter. The DNA–DNA relatedness values of this strain to strains SH-1Tand IMCC1728Twere only 35.4 and 33.3 %, respectively. Cells of MQ-18Twere Gram-negative, aerobic, motile, rod-shaped and non-spore forming. This strain exhibited growth at 25–37 °C (optimum: 30 °C) in the presence of 0–3.0 % (w/v) NaCl (optimum, 0 % NaCl) and at pH 5.0–8.0 (pH 7.0). The predominant fatty acids were C12 : 0(5.5 %), C16 : 0(33.7 %), summed feature 3 (C16 : 1ω7cand/or C16 : 1ω6c; 38.5 %), and summed feature 4 (anteiso-C17 : 1B and/or iso C17 : 1I; 11.6 %). The main quinone type was ubiquinone-8, and the major polyamines were cadaverine and putrescine. The major polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content was 70.1 mol%. On the basis of its phylogenetic, phenotypic and physiological characteristics, strain MQ-18Tis considered to represent a novel species of the genusPiscinibacter, for which the namePiscinibacter caenisp. nov. is proposed. The type strain is MQ-18T(CCTCC AB 2017223T=JCM 32138T).