A NOVEL LIGAND FOR CD44 IS SERGLYCIN, A HEMATOPOIETIC-CELL LINEAGE-SPECIFIC PROTEOGLYCAN - POSSIBLE INVOLVEMENT IN LYMPHOID-CELL ADHERENCE AND ACTIVATION
A NOVEL LIGAND FOR CD44 IS SERGLYCIN, A HEMATOPOIETIC-CELL LINEAGE-SPECIFIC PROTEOGLYCAN - POSSIBLE INVOLVEMENT IN LYMPHOID-CELL ADHERENCE AND ACTIVATION
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DOI：
10.1074/jbc.270.13.7437
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发表时间：
1995-03-31
影响因子：
4.8
通讯作者：
MIYASAKA, M
中科院分区：
文献类型：
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作者：
TOYAMASORIMACHI, N;SORIMACHI, H;MIYASAKA, M
淋巴细胞粘附分子CD44识别由小鼠T细胞系CTLL 2分泌的非透明质酸蛋白聚糖gp600。我们现在证明，gp600是相同的serglycin，小蛋白聚糖家族的成员存储在细胞内分泌颗粒的淋巴，骨髓，和一些肿瘤细胞。纯化的gp600具有与CD44特异性结合的能力，并且这种结合依赖于CD44的活化。gp600或sergycin上的CD44结合元件是由4-硫酸软骨素组成的糖胺聚糖。丝甘肽易于胞吐，并且其与活性形式CD44的相互作用增强CD44阳性CTL克隆的CDS依赖性脱粒。结论：造血细胞分泌颗粒分泌的sergylycin是一种新的CD44配体，可调节淋巴细胞的粘附和活化。
The lymphocyte adhesion molecule CD44 recognizes a non-hyaluronate proteoglycan, gp600, secreted by mouse T cell line CTLL2. We now demonstrate that gp600 is identical to serglycin, a member of the small proteoglycan family stored in intracellular secretory granules of lymphoid, myeloid, and some tumor cells. Purified gp600 has the ability to bind specifically to CD44, and the binding is dependent on activation of CD44. The CD44-binding elements on gp600 or serglycin are glycosaminoglycans consisting of chondroitin 4-sulfate. Serglycin is readily exocytosed, and its interaction with active form CD44 augments the CDS dependent degranulation of CD44 positive CTL clones. We conclude that the serglycin secreted from secretory granules of hematopoietic cells is a novel ligand for CD44, and could regulate lymphoid cell adherence and activation.