Factors affecting stability and infectivity of SARS-CoV-2
Factors affecting stability and infectivity of SARS-CoV-2
复制标题
DOI：
10.1016/j.jhin.2020.07.009
复制
发表时间：
2020-10-01
影响因子：
6.9
通讯作者：
Yuen, K-Y
中科院分区：
文献类型：
--
作者：
Chan, K-H;Sridhar, S.;Yuen, K-Y
背景：2019年底，一种新型人类冠状病毒-严重急性呼吸综合征冠状病毒-2（SARS-CoV-2）-在中国武汉出现。这种病毒已造成全球大流行，涉及200多个国家。SARS-CoV-2是一种高度适应人类的病毒，在人与人之间传播非常迅速。目的：研究SARS-CoV-2在不同环境和pH条件下的感染性。方法：采用不同的实验室病毒灭活方法和家用消毒剂，分别于0、1、3、5、7 d对Vero E6细胞进行病毒灭活试验。在室温（20-25摄氏度）下用各种消毒剂和pH溶液处理后测定病毒活力。结果：SARS-CoV-2能够在室温下以干燥形式保留活力3-5天或在溶液中保留活力7天。SARS-CoV-2可在pH 4至pH 11的广泛pH条件下检测数天，室温下在粪便中检测1-2天，但失去5个对数的感染性。各种常用的消毒剂和实验室灭活程序被发现，以减少病毒violence.Conclusion：这项研究表明，SARS冠状病毒-2在环境表面的稳定性，并提出了粪-口传播的可能性。研究发现，常用的固定剂、核酸提取方法和热灭活方法可显著降低病毒感染性，这可以确保SARS-CoV-2大流行期间医院和实验室的安全。(C)2020年，任作家。由Elsevier Ltd代表The Healthcare Infection Society出版。
Background: In late 2019, a novel human coronavirus - severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) - emerged in Wuhan, China. This virus has caused a global pandemic involving more than 200 countries. SARS-CoV-2 is highly adapted to humans and readily transmits from person -to -person.Aim: To investigate the infectivity of SARS-CoV-2 under various environmental and pH conditions. The efficacies of various laboratory virus inactivation methods and home disinfectants against SARS-CoV-2 were investigated.Methods: The residual virus in dried form or in solution was titrated on to Vero E6 cells on days 0, 1, 3, 5 and 7 after incubation at different temperatures. Viral viability was determined after treatment with various disinfectants and pH solutions at room temperature (20-25 degrees C).Findings: SARS-CoV-2 was able to retain viability for 3-5 days in dried form or 7 days in solution at room temperature. SARS-CoV-2 could be detected under a wide range of pH conditions from pH 4 to pH 11 for several days, and for 1-2 days in stool at room temperature but lost 5 logs of infectivity. A variety of commonly used disinfectants and laboratory inactivation procedures were found to reduce viral viability effectively.Conclusion: This study demonstrated the stability of SARS-CoV-2 on environmental surfaces, and raises the possibility of faecal-oral transmission. Commonly used fixatives, nucleic acid extraction methods and heat inactivation were found to reduce viral infectivity significantly, which could ensure hospital and laboratory safety during the SARS-CoV-2 pandemic. (C) 2020 The Authors. Published by Elsevier Ltd on behalf of The Healthcare Infection Society.