Heparin-like polymers modulate proinflammatory cytokine production by lipopolysaccharide-stimulated human monocytes
Heparin-like polymers modulate proinflammatory cytokine production by lipopolysaccharide-stimulated human monocytes
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DOI：
10.1002/jbm.10112
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发表时间：
2002-06-05
期刊：
影响因子：
--
通讯作者：
Letourneur, D
中科院分区：
文献类型：
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作者：
Anastase-Ravion, S;Carreno, MP;Letourneur, D
寻找类似肝素的材料仍然是一个密集的研究领域。在此背景下，我们研究了半合成葡聚糖衍生物和棕色海藻(岩藻多糖)中天然存在的硫酸盐多糖的免疫调节特性。在这项研究中，我们通过分析它们对静息或脂多糖(LPS)刺激的人单核细胞释放促炎细胞因子的影响以及它们与单核细胞表面的相互作用来研究它们的功能潜力。结果表明，葡聚糖、葡聚糖衍生物和肝素分别以剂量依赖的方式刺激单核细胞产生IL-let、肿瘤坏死因子α、IL-6和IL-8，(2)调节内毒素刺激的单核细胞产生细胞因子，(3)特异性地抑制生物素化的内毒素与单核细胞膜的结合。综上所述，这些数据表明，岩藻糖胶和葡聚糖衍生物对人体血细胞具有有趣的免疫调节作用，这可能与新药或生物材料涂层有关。事实上，这种多糖通过调节单核细胞的激活，可以帮助改善植入物的生物相容性。(C)2002年威利期刊公司。
The search for heparin-like materials remains an intensive field of research. In this context, we studied the immunomodulatory properties of semisynthetic dextran derivatives and naturally occurring sulfated polysaccharides present in brown seaweed (fucans). In this study, we investigated the functional potencies of fucan and dextran derivatives by analyzing their effects on the release of proinflammatory cytokines by resting or lipopolysaccharide (LPS)stimulated human monocytes and their interactions on monocyte surfaces. The results showed that fucan, dextran derivatives, and heparin differentially (1) triggered interleukin-let, tumor necrosis factor alpha, interleukin-6, and interleukin-8 production by monocytes in a dose-dependent manner, (2) modulated cytokine production by LPS-stimulated monocytes, and (3) specifically inhibited the binding of biotinylated LPS to monocyte membranes. Taken together, these data indicated that fucan and dextran derivatives displayed interesting immunomodulatory effects on human blood cells that could be relevant as new drugs or biomaterial coatings. Indeed, such polysaccharides, by regulating monocyte activation, could contribute to the improved biocompatibility of implants. (C) 2002 Wiley Periodicals, Inc.