Growth-promoting effects of acidic and basic fibroblast growth factor on rabbit articular chondrocytes aging in culture.
Growth-promoting effects of acidic and basic fibroblast growth factor on rabbit articular chondrocytes aging in culture.
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酸性和碱性成纤维细胞生长因子对培养的兔关节软骨细胞老化的促生长作用。
DOI：
10.1016/0014-4827(89)90399-6
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发表时间：
1989
影响因子：
3.7
通讯作者：
M. Adolphe
中科院分区：
文献类型：
--
作者：
B. Froger‐Gaillard;A. Charrier;S. Thenet;X. Ronot;M. Adolphe
兔关节软骨细胞在体外生长能力有限。经过四次传代培养，软骨细胞已经完成了超过50%的寿命。在培养的这个阶段，它们被认为是衰老样的，因为观察到增殖能力急剧下降，细胞大小和蛋白质含量增加。然而，这些老化的细胞仍然能够对成纤维细胞生长因子（FGF）作出反应。向培养基中添加酸性或碱性FGF（10 ng/ml）允许增强的增殖。在老化过程中FGF促有丝分裂活性的衰减没有观察到这两个馏分。此外，当用酸性或碱性FGF处理时，老化的软骨细胞具有较小的尺寸和较低的蛋白质含量。酸性FGF在延缓老化软骨细胞向衰老的演变方面不如碱性FGF有效。
Rabbit articular chondrocytes have a limited growth potentialin vitro. After four passages in culture, chondrocytes have accomplished more than 50% of their life span. At this stage of culture, they are considered to be senescent-like, since a dramatic decrease in proliferative capacity and enhanced cell size and protein content are observed. These aged cells are, however, still able to respond to fibroblast growth factor (FGF). The addition of either acidic or basic FGF (10 ng/ml) to culture medium permitted an enhanced proliferation. The attenuation of FGF mitogenic activity during aging was not observed for both fractions. Moreover, when treated with acidic or basic FGF, aged chondrocytes had a smaller size and a lower protein content. The acidic FGF was less potent than the basic FGF in delaying the evolution of aged chondrocytes to senescence.