Overexpression of (At)NPR1 in rice leads to a BTH- and environment-induced lesion-mimic/cell death phenotype
Overexpression of (At)NPR1 in rice leads to a BTH- and environment-induced lesion-mimic/cell death phenotype
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DOI：
10.1094/mpmi.2004.17.2.140
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发表时间：
2004-02-01
影响因子：
3.5
通讯作者：
Ronald, PC
中科院分区：
文献类型：
--
作者：
Fitzgerald, HA;Chern, MS;Ronald, PC
系统获得抗性（SAR）是一种可诱导的防御反应，保护植物免受广谱病原体的侵害。拟南芥中SAR的一个中心调节因子是NPR 1（nonexpresser of pathogenesis-related genes）。在水稻中，拟南芥NPR 1的过表达增强了植物对细菌病原体黄单胞菌的抗性。米。这份报告表明，（At）NPR 1在水稻中的过表达也触发了病变模拟/细胞死亡（LMD）表型。LMD表型是环境调节和遗传的。此外，病害的发生和死亡与水稻防御基因的表达和过氧化氢的积累有关。水杨酸（SA）类似物苯并（1，2，3）噻二唑-7-硫代羧酸S-甲酯（BTH）的应用增强了这种表型。与野生型植物相比，在过表达（At）NPR 1的水稻中内源SA水平降低，这支持了（At）NPR 1可能感知和调节SA积累的观点。水稻中（At）NPR 1表达与LMD表型的发展的关联表明（At）NPR 1在植物胁迫反应中具有多种作用，这可能影响其作为工程广谱抗性的转基因工具的功效。
Systemic acquired resistance (SAR) is an inducible defense response that protects plants against a broad spectrum of pathogens. A central regulator of SAR in Arabidopsis is NPR1 (nonexpresser of pathogenesis-related genes). In rice, overexpression of Arabidopsis NPR1 enhances plant resistance to the bacterial pathogen Xanthomonas oryzae pv. oryzae. This report demonstrates that overexpression of (At)NPR1 in rice also triggers a lesion-mimic/cell death (LMD) phenotype. The LMD phenotype is environmentally regulated and heritable. In addition, the development of lesions and death correlates with the expression of rice defense genes and the accumulation of hydrogen peroxide. Application of the salicylic acid (SA) analog, benzo(1,2,3) thiadiazole-7-carbothioc acid S-methyl ester (BTH), potentiates this phenotype Endogenous SA levels are reduced in rice overexpressing (At)NPR1 when compared with wildtype plants, supporting the idea that (At)NPR1 may perceive and modulate the accumulation of SA. The association of (At)NPR1 expression in rice with the development of an LMD phenotype suggests that (At)NPR1 has multiple roles in plant stress responses that may affect its efficacy as a transgenic tool for engineering broad-spectrum resistance.