GENETIC-CONTROL OF BUD SITE SELECTION IN YEAST BY A SET OF GENE-PRODUCTS THAT CONSTITUTE A MORPHOGENETIC PATHWAY
GENETIC-CONTROL OF BUD SITE SELECTION IN YEAST BY A SET OF GENE-PRODUCTS THAT CONSTITUTE A MORPHOGENETIC PATHWAY
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DOI：
10.1016/0092-8674(91)90015-q
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发表时间：
1991-06-28
期刊：
影响因子：
64.5
通讯作者：
HERSKOWITZ, I
中科院分区：
文献类型：
--
作者：
CHANT, J;HERSKOWITZ, I
酵母细胞在其表面上以两种不同的空间模式选择芽位点：a和α-细胞为轴向，a/α-细胞为双极。我们已经确定了四个基因，BUD 1-BUD 4，必要的轴向模式，通过分离突变体的α细胞，不表现出这种模式。BUD 1（与先前鉴定的基因RSR 1相同）或BUD 2中的突变导致所有细胞类型中的随机出芽模式; BUD 3或BUD 4中的突变导致所有细胞类型中的双极模式。这些观察结果表明，存在一个基础的萌芽模式，不需要BUD产品，这是随机的; BUD 1和BUD 2的作用，在这个基础模式，以创建双极模式; BUD 3和BUD 4的进一步行动，导致轴向模式。因此，这些研究确定了一组基因产物，指导细胞形态发生的遗传编程的网站。
Yeast cells choose bud sites on their surface in two distinct spatial patterns: axial for a and alpha-cells and bipolar for a/alpha-cells. We have identified four genes, BUD1-BUD4, necessary for the axial pattern by isolating mutants of alpha-cells that do not exhibit this pattern. Mutations in BUD1 (which is the same as the previously identified gene RSR1) or BUD2 lead to a random budding pattern in all cell types; mutations in BUD3 or BUD4 lead to a bipolar pattern in all cell types. These observations indicate the existence of a basal budding pattern, requiring no BUD products, that is random; BUD1 and BUD2 act on this basal pattern to create the bipolar pattern; the further action of BUD3 and BUD4 leads to the axial pattern. These studies thus identify a set of gene products that directs cell morphogenesis to a genetically programmed site.