Thiosulfate in urine as a facilitator in the diagnosis of prostate cancer for patients with prostate-specific antigen less or equal 10 ng/mL
Thiosulfate in urine as a facilitator in the diagnosis of prostate cancer for patients with prostate-specific antigen less or equal 10 ng/mL
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DOI：
10.1515/cclm-2013-0069
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发表时间：
2013-09-01
影响因子：
6.8
通讯作者：
Krzeslak, Anna
中科院分区：
文献类型：
--
作者：
Chwatko, Grazyna;Forma, Ewa;Krzeslak, Anna
背景资料：本研究的目的是检测前列腺癌（PCa）患者尿液中硫代硫酸盐的水平，并评估其在诊断和监测前列腺恶性转化中的作用。硫代硫酸盐是硫化氢（H2S）代谢的天然产物。方法：采用反相液相色谱法，以2-氯-1-甲基喹啉四氟硼酸盐为衍生试剂，测定前列腺癌患者和健康对照者尿液中硫代硫酸盐的含量。硫代硫酸盐浓度归一化为尿肌酐水平，以补偿变量diurement.Results：在前列腺癌患者的尿液样本中，平均硫代硫酸盐水平几乎是50倍高于对照组和5倍高于良性前列腺增生组。硫代硫酸盐水平与血清前列腺特异性抗原（PSA）水平或PSA密度无关。肿瘤分期和肿瘤级别与硫代硫酸盐水平均不相关。结论：结果表明尿液中硫代硫酸盐浓度可能是前列腺癌诊断的良好促进因素。该方法的预测准确性对血清PSA水平低且直肠指检和经直肠超声结果阴性的患者的诊断特别有价值。
Background: The aim of this study was to examine the level of thiosulfate in the urine of prostate cancer (PCa) patients and evaluate its usefulness in the diagnosis and monitoring of prostate malignant transformation. Thiosulfate is a naturally occurring product of hydrogen sulfide (H2S) metabolism. H2S is involved in many physiological and pathological processes including inflammation and tumorigenesis.Methods: The determination of thiosulfate in the urine of PCa patients and healthy controls was performed by reverse-phased liquid chromatography using 2-chloro-1-methylquinolinium tetrafluoroborate as a derivatization reagent. Thiosulfate concentrations were normalized to urinary creatinine levels to compensate for variable diuresis.Results: In the urine samples of PCa patients, the mean thiosulfate level was almost 50 times higher than in the control groups and five times higher than in the benign prostatic hyperplasia group. The level of thiosulfate did not correlate with the serum prostate-specific antigen (PSA) level or PSA density. Neither tumor stage nor tumor grade was associated with thiosulfate level.Conclusions: The results suggest that thiosulfate concentration in urine may be a good facilitator in the diagnostics of PCa. The predictive accuracy of this method is particularly valuable for the diagnosis of patients with low serum PSA level and negative digital rectal examination and transrectal ultrasound results.