Outdoor H2 production in a 50-L tubular photobioreactor by means of a sulfur-deprived culture of the microalga Chlamydomonas reinhardtii
Outdoor H2 production in a 50-L tubular photobioreactor by means of a sulfur-deprived culture of the microalga Chlamydomonas reinhardtii
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DOI：
10.1016/j.jbiotec.2011.06.040
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发表时间：
2012-02-20
影响因子：
4.1
通讯作者：
Torzillo, Giuseppe
中科院分区：
文献类型：
--
作者：
Scoma, Alberto;Giannelli, Luca;Torzillo, Giuseppe
在过去的十年中，H-2的生产使用绿色microenvironment莱茵衣藻已被广泛研究实验室规模的光生物反应器，而户外文化的信息仍然缺乏。本文研究了缺硫条件下的C. reinhardtii在50-L水平管式光生物反应器中进行的光降解试验。在人工和直接太阳光下进行了制氢实验。在这两种情况下，获得的H-2产量是实验室中获得的产量的18-20%。然而，在实验室条件下测试室外生长的细胞时，没有观察到H-2生产的显着变化。叶绿素荧光测量表明，户外培养受到强烈的光抑制，由于高太阳光强度和硫剥夺的组合。事实上，H-2的生产只有在户外实现的文化是以前适应阳光下，一个条件，导致了一些生理变化，即：（i）减少叶绿素含量每单位干重;（ii）增加光合作用和呼吸速率，和（iii）较高的诱导叶黄素循环色素相比，非适应的文化。得出的结论是，在50-L光生物反应器中实现的H-2输出减少是由于培养物暴露于不同的照明模式（实验室中提供的单侧照明与双侧照明），以及混合时间的巨大差异（实验室规模光生物反应器中实现的60分钟与15.5秒）。据我们所知，这是第一次通过太阳光实现用绿色藻类生产H-2。(C)2011爱思唯尔有限公司版权所有。
In the past decade, H-2 production using the green microalga Chlamydomonas reinhardtii has been extensively studied under laboratory-scale photobioreactors, while information on outdoor cultures is still lacking. In this paper, the results of experiments conducted with sulfur-deprived cultures of C. reinhardtii carried out in a 50-L horizontal tubular photobioreactor are presented. Hydrogen production experiments were carried out under both artificial and direct solar light. In both cases, the H-2 output attained was 18-20% of what obtained in the laboratory. However, no significant changes in the H-2 production were observed when cells grown outdoors were tested under laboratory conditions. Chlorophyll fluorescence measurements showed that outdoor cultures were subjected to strong photo-inhibition, due to the combination of high solar light intensity and sulfur-deprivation. Indeed, H-2 production was only achieved outdoors when cultures were previously acclimated to sunlight, a condition that caused a number of physiological changes, namely: (i) a decrease in the chlorophyll content per unit of dry weight: (ii) an increase in the photosynthesis and respiration rates, and (iii) a higher induction of the xanthophyll cycle pigments as compared to non-acclimated cultures. It was concluded that the reduced H-2 output achieved in the 50-L photobioreactor was due to the different illumination pattern to which the cultures were exposed (one-sided vs. two-sided illumination provided in the laboratory), as well as to the great difference in the mixing times (60 min vs. 15.5 s achieved in the lab-scale photobioreactor). To the very best of our knowledge this is the first time that H-2 production with green algae has been achieved by means of solar light. (C) 2011 Elsevier B.V. All rights reserved.