[Emerging of avian leukosis virus subgroup J in a flock of Chinese local breed].
[Emerging of avian leukosis virus subgroup J in a flock of Chinese local breed].
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发表时间：
2005-08
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通讯作者：
Ziqiang Cheng;Li Zhang;Si-dang Liu;Lingjuan Zhang;Z. Cui
中科院分区：
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作者：
Ziqiang Cheng;Li Zhang;Si-dang Liu;Lingjuan Zhang;Z. Cui
髓性白血病（ML）病例首次在山东省的中国地方品种鸡群中被诊断出来。主要症状为消瘦、体重减轻、贫血。它导致约15000只120天大的鸟死亡，死亡率约为10%。尸检可见胸骨、肠、气管表面常见大小不一的灰白色结节和突出物。几乎所有脏器组织均有中度至重度肿大，弥漫性灰白色结节。组织学检查显示，组织内增生的肿瘤细胞为髓细胞，胞浆内有嗜酸性颗粒。用一对alv - j特异性引物进行PCR， 17份肝脏样本中有15份呈阳性。对1份阳性样品的PCR产物进行测序，在核酸和氨基酸水平上与ALV-J HPRS-103的同源性分别为98.05%和97.4%。用ALV-J单克隆抗体免疫组化（IHC）技术检测，肿瘤组织、肝、脾、肾、骨髓和前脑室染色最强烈。结果表明，ALV-J在中国地方品种中已引起鸡感染和死亡。
Myeloid leukosis (ML) cases were first diagnosed in a chicken flock of Chinese local breed in Shan dong province. The main symptom included wasting, weight loss, anemia. It caused about 10% mortality of about 15000 birds at the age of 120-day. In the necropsy, gray-white nodules and protrusions in various sizes were commonly observed on the surface of the sternum, intestine and trachea. Almost all viscera tissues showed moderate to severe enlargement with diffuse gray-white nodules. Histological examination indicated that the tumor cells proliferated in tissues were myelocytes with eosinophilic granules in cytoplasm. In PCR with a pair of ALV-J-specific primers, 15 of 17 liver samples were positive. PCR product of one positive sample was sequenced and demonstrated 98.05% and 97.4% identity with ALV-J HPRS-103 strain at nuclei acid and amino acids level, respectively. By immunohistochemistry (IHC) technique with ALV-J monoclonal antibody, the most intense staining was in the tumor tissue, liver, spleen, kidney, bone marrow, and proventriculus. The results indicate that ALV-J already caused chickens infection and dead in Chinese local breed.