Corrigendum: The seco-iridoid pathway from Catharanthus roseus
Corrigendum: The seco-iridoid pathway from Catharanthus roseus
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CRRIGENDUM：Catharanthus Roseus的Seco-Iridoid途径
DOI：
10.1038/ncomms5175
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发表时间：
2014-06-24
影响因子：
16.6
通讯作者：
Werck-Reichhart D
中科院分区：
文献类型：
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作者：
Miettinen K;Dong L;Navrot N;Schneider T;Burlat V;Pollier J;Woittiez L;van der Krol S;Lugan R;Ilc T;Verpoorte R;Oksman-Caldentey KM;Martinoia E;Bouwmeester H;Goossens A;Memelink J;Werck-Reichhart D
The (seco) iridoids and their derivatives, the monoterpenoid indole alkaloids (MIAs), form two large families of plant-derived bioactive compounds with a wide spectrum of high-value pharmacological and insect-repellent activities. Vinblastine and vincristine, MIAs used as anti-cancer drugs, are produced by Catharanthus roseus in extremely low levels, leading to high market prices and poor availability. Their biotechnological production is hampered by the fragmentary knowledge of their biosynthesis. Here we report the discovery of the last four missing steps of the (seco) iridoid biosynthesis pathway. Expression of the eight genes encoding this pathway together with two genes boosting precursor formation and two downstream alkaloid biosynthesis genes in an alternative plant host allowed the heterologous production of the complex MIA strictosidine. This confirms the functionality of all enzymes of the pathway and highlights their utility for synthetic biology programs towards a sustainable biotechnological production of valuable (seco) iridoids and alkaloids with pharmaceutical and agricultural applications.
影响因子：
16.6
作者：
Miettinen, Karel;Dong, Lemeng;Navrot, Nicolas;Schneider, Thomas;Burlat, Vincent;Pollier, Jacob;Woittiez, Lotte;van der Krol, Sander;Lugan, Raphael;Ilc, Tina;Verpoorte, Robert;Oksman-Caldentey, Kirsi-Marja;Martinoia, Enrico;Bouwmeester, Harro;Goossens, Alain;Memelink, Johan;Werck-Reichhart, Daniele
通讯作者：
Werck-Reichhart, Daniele