Uniform-sized molecularly imprinted polymer for (S)-naproxen selectively modified with hydrophilic external layer
Uniform-sized molecularly imprinted polymer for (S)-naproxen selectively modified with hydrophilic external layer
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DOI：
10.1016/s0021-9673(99)00570-1
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发表时间：
1999-07-23
影响因子：
4.1
通讯作者：
Tanaka, N
中科院分区：
文献类型：
--
作者：
Haginaka, J;Takekira, H;Tanaka, N
制备了一种亲水性外层修饰的（S）-萘普生选择性分子印迹聚合物。首先，分别以4-乙烯基吡啶和乙二醇二甲基丙烯酸酯（EDMA）为功能单体和交联剂，采用多步溶胀热聚合法制备了（S）-萘普生分子印迹聚合物。接着，将单甲基丙烯酸甘油酯（GMMA）和二甲基丙烯酸甘油酯（GDMA）的1：1混合物用于亲水性表面改性，并且在分子印迹开始后4 h将其直接添加到（S）-萘普生的分子印迹聚合物中。与未改性的分子印迹聚合物相比，表面改性的分子印迹聚合物对所有被测溶质的保留因子均有所降低。然而，外消旋萘普生的手性识别与表面改性的分子印迹聚合物以及未改性的分子印迹聚合物。此外，牛血清白蛋白从表面改性的分子印迹聚合物中完全回收。结果表明，（S）-萘普生分子印迹聚合物表面亲水修饰后，其手性识别位点没有发生变化，亲水外层对（S）-萘普生分子印迹聚合物进行了选择性修饰。初步结果表明，表面修饰的分子印迹聚合物可适用于直接血清注射检测（S）-萘普生。(C)1999 Elsevier Science B. V.保留所有权利。
A uniform-sized molecularly imprinted polymer (MIP) for (S)-naproxen selectively modified with hydrophilic external layer has been prepared. First, the molecularly imprinted polymer for (S)-naproxen was prepared using 4-vinylpyridine and ethylene glycol dimethacrylate (EDMA) as a functional monomer and cross-linker, respectively, by a multi-step swelling and thermal polymerization method. Next, a 1:1 mixture of glycerol monomethacrylate (GMMA) and glycerol dimethacrylate (GDMA) was used for hydrophilic surface modification, and it was added directly to the molecularly imprinted polymer for (S)-naproxen 4 h after the start of molecular imprinting. The retention factors of all solutes tested were decreased with the surface modified molecularly imprinted polymer, compared with the unmodified molecularly imprinted polymer. However, chiral recognition of racemic naproxen was attained with the surface modified molecularly imprinted polymer as well as the unmodified molecularly imprinted polymer. Further, bovine serum albumin was completely recovered from the surface modified molecularly imprinted polymer. These results revealed that the chiral recognition sites of (S)-naproxen remained unchanged with hydrophilic surface modification, and that the molecularly imprinted polymer for (S)-naproxen was selectively modified with hydrophilic external layer. Preliminary results reveal that the surface modified molecularly imprinted polymer could be applicable to direct serum injection assays of (S)-naproxen. (C) 1999 Elsevier Science B.V. All rights reserved.