Saccharomyces boulardii decreases inflammation and intestinal colonization by Candida albicans in a mouse model of chemically-induced colitis
Saccharomyces boulardii decreases inflammation and intestinal colonization by Candida albicans in a mouse model of chemically-induced colitis
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DOI：
10.1080/13693780701523013
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发表时间：
2007-12-01
期刊：
影响因子：
2.9
通讯作者：
Poulain, Daniel
中科院分区：
文献类型：
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作者：
Jawhara, Samir;Poulain, Daniel
本研究旨在研究布拉氏酵母菌对右旋糖酐硫酸钠 (DSS) 诱导的 BALB/c 小鼠结肠炎模型中炎症和白色念珠菌肠道定植的影响。通过口服管饲107个酵母细胞的200μL悬浮液来建立白色念珠菌的定植。在白色念珠菌口服攻击后1小时，将1.5％的DSS溶液加入饮用水中，同时每天通过口服管饲法接种107个布拉氏酵母菌细胞，持续1周。连续两周每天收集粪便。 14 天后，由给予白色念珠菌或布拉氏链球菌或两者的小鼠组成的七组小鼠被处死，并采集结肠样本进行组织学评分以及炎症细胞因子和 Toll 样受体 (TLR) 的实时 PCR (RT-PCR) 分析。与未接受 DSS 的对照动物相比，接受 DSS 的小鼠从粪便中回收的白色念珠菌菌落数量明显较多。相比之下，接受布拉氏酵母菌治疗的小鼠中，白色念珠菌的菌落形成单位（CFU）大大减少。使用这种酵母可以降低 DSS 诱导的临床评分和组织学炎症的严重程度。在 mRNA 表达水平上，布拉氏酵母菌的存在导致 TLR2 和 TLR4 的增加与炎症细胞因子 TNF α 和 INF γ 的减少相关。在接受 DSS 和白色念珠菌的小鼠中，两种酵母刺激后 TLR4 过度表达，但单独施用白色念珠菌时增加的 TLR2 和 TNF α 在布拉氏酵母菌存在时减少。这些结果表明，布拉氏酵母菌减少了 BALB/c 小鼠结肠炎模型中的炎症和白色念珠菌定植。
The present study was designed to investigate the effects of Saccharomyces boulardii on inflammation and intestinal colonization by Candida albicans in a BALB/c mouse model of colitis that had been induced by dextran-sulfate-sodium (DSS). Colonization with C albicans was established by oral gavage with a 200 mu L suspension of 107 yeast cells. A 1.5% solution of DSS was administered in drinking water I h after C albicans oral challenge, while 107 cells of S. boulardii was inoculated daily by oral gavage for I week. Faeces were collected daily for 2 weeks. Seven groups of mice consisting of those that were administered either C. albicans or S. boulardii or both were sacrificed after 14 days and samples of the colon were taken for histological scoring and real-time PCR (RT-PCR) analysis of inflammatory cytokines and toll-like receptors (TLRs). Compared to control animals that did not receive DSS, the number of C albicans colonies recovered from faeces was significantly greater in mice receiving DSS. In contrast, the colony forming units (CFUs) of C albicans were greatly reduced in mice receiving S. boulardii. The administration of this yeast decreased the severity of DSS-induced clinical scores and histological inflammation. At the mRNA expression level, an increase in TLR2 and TLR4 resulting from the presence of S. boulardii was associated with a reduction in the inflammatory cytokines TNF alpha and INF gamma. In mice receiving DSS and C albicans, TLR4 was over-expressed by stimulation with both yeasts, but TLR2 and TNF alpha which were increased by the administration of C albicans alone, were decreased in the presence of S. boulardii. These results indicate that S. boulardii decreased inflammation and C albicans colonization in this BALB/c mouse model of colitis.