Methodological considerations for meal-induced thermogenesis: measurement duration and reproducibility
Methodological considerations for meal-induced thermogenesis: measurement duration and reproducibility
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DOI：
10.1017/s0007114513001451
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发表时间：
2013-12-14
影响因子：
3.6
通讯作者：
Wood，Rachel E.
中科院分区：
文献类型：
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作者：
Ruddick-Collins，Leonie C.;King，Neil A.;Wood，Rachel E.
膳食产热（MIT）的研究结果一直高度不一致，部分原因是由于测量MIT的持续时间和方案的不同。在本研究中，我们旨在确定以下内容：(1)在3,4,5小时完成6小时MIT反应的比例；(2)较短持续时间与6小时测量之间的关联；(3)较短的持续时间是否提高了测量的再现性。研究人员对10个人（5男5女）在两次进食2410kj的混合膳食后进行了MIT测量。用间接量热法连续测量餐后6小时的能量消耗，计算MIT为餐前RMR以上能量消耗的增加。平均而言，76%、89%和96%的6小时MIT反应分别在3、4和5小时内完成，这些时间点的MIT与6小时MIT反应呈强相关（相关范围为0.990 - 0.998；P< 0.01）。测量6 h时的日间CV值为33%，测量3 h后显著降低（CV值为26%，P= 0.02）。尽管不同天数的MIT总量存在差异，但在3,4和5h完成的MIT比例是可重复的（平均CV: 5%）。虽然通常需要6小时来测量完整的MIT反应，但3小时的测量提供了关于MIT反应大小的足够信息，并且可能适用于重复场合的个体测试。
Meal-induced thermogenesis (MIT) research findings have been highly inconsistent, in part, due to the variety of durations and protocols used to measure MIT. In the present study, we aimed to determine the following: (1) the proportion of a 6 h MIT response completed at 3, 4 and 5 h; (2) the associations between the shorter durations and the 6 h measures; (3) whether shorter durations improved the reproducibility of the measurement. MIT was measured in response to a 2410 kJ mixed composition meal in ten individuals (five males and five females) on two occasions. Energy expenditure was measured continuously for 6 h post-meal using indirect calorimetry, and MIT was calculated as the increase in energy expenditure above the pre-meal RMR. On average, 76, 89 and 96 % of the 6 h MIT response was completed within 3, 4 and 5 h, respectively, and MIT at each of these time points was strongly correlated with the 6 h MIT response (range for correlations, r 0·990–0·998; P< 0·01). The between-day CV for the 6 h measurement was 33 %, but it was significantly lower after 3 h of measurement (CV 26 %; P= 0·02). Despite variability in the total MIT between days, the proportion of MIT that was completed at 3, 4 and 5 h was reproducible (mean CV: 5 %). While 6 h are typically required to measure the complete MIT response, the 3 h measures provide sufficient information about the magnitude of the MIT response and may be applicable for testing individuals on repeated occasions.