The 'mustard oil bomb': not so easy to assemble?! Localization, expression and distribution of the components of the myrosinase enzyme system
The 'mustard oil bomb': not so easy to assemble?! Localization, expression and distribution of the components of the myrosinase enzyme system
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DOI：
10.1007/s11101-008-9109-1
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发表时间：
2009-01-01
影响因子：
7.7
通讯作者：
Bones, Atle M.
中科院分区：
文献类型：
--
作者：
Kissen, Ralph;Rossiter, John T.;Bones, Atle M.
硫代葡萄糖苷是植物次生代谢物，通过黑芥子酶的作用水解成各种产物（异硫氰酸酯、硫氰酸酯、上皮腈、腈、恶唑烷）。硫代葡萄糖苷的大量水解只发生在组织损伤时，但也有证据表明硫代葡萄糖苷在完整的植物组织中也有代谢。人们最初认为，完整植物中的硫代葡萄糖苷-黑芥子酶系统是稳定的，这是由于其组分在空间上的分离。这被称为“芥末油炸弹”理论。与黑芥子酶形成复合物的蛋白质有：黑芥子酶结合蛋白（MBPs）和黑芥子酶相关蛋白（MyAPs/ESM）。这些蛋白的作用及其生物学意义尚不完全清楚。黑芥子酶系统的其他蛋白质是上皮指示蛋白（ESP）和硫氰酸酯形成蛋白（TFP），它们将硫代葡萄糖苷水解从异硫氰酸酯生产转移到腈/上皮腈或硫氰酸酯生产。一些硫代葡萄糖苷水解产物作为植物对昆虫和病原体的防御化合物或对人体有益的健康影响。在这篇综述中，我们调查和批判性地评估有关定位的现有信息，在组织/细胞和亚细胞水平，黑芥子酶系统的不同组成部分。来自模式植物拟南芥的数据与来自其他产硫代葡萄糖苷的十字花科植物的数据进行了比较，以显示这些物种中“芥菜油炸弹”的共同和不同特征。
Glucosinolates are plant secondary metabolites that are hydrolysed by the action of myrosinases into various products (isothiocyanates, thiocyanates, epithionitriles, nitriles, oxazolidines). Massive hydrolysis of glucosinolates occurs only upon tissue damage but there is also evidence indicating metabolism of glucosinolates in intact plant tissues. It was originally believed that the glucosinolate-myrosinase system in intact plants was stable due to a spatial separation of the components. This has been coined as the 'mustard oil bomb' theory. Proteins that form complexes with myrosinases have been described: myrosinase-binding proteins (MBPs) and myrosinase-associated proteins (MyAPs/ESM). The roles of these proteins and their biological relevance are not yet completely known. Other proteins of the myrosinase enzyme system are the epithiospecifier protein (ESP) and the thiocyanate-forming protein (TFP) that divert the glucosinolate hydrolysis from isothiocyanate production to nitrile/epithionitrile or thiocyanate production. Some glucosinolate hydrolysis products act as plant defence compounds against insects and pathogens or have beneficial health effects on humans. In this review, we survey and critically assess the available information concerning the localization, both at the tissular/cellular and subcellular level, of the different components of the myrosinase enzyme system. Data from the model plant Arabidopsis thaliana is compared to that from other glucosinolate-producing Brassicaceae in order to show common as well as divergent features of the 'mustard oil bomb' among these species.