4D microvascular imaging based on ultrafast Doppler tomography
4D microvascular imaging based on ultrafast Doppler tomography
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DOI：
10.1016/j.neuroimage.2015.11.014
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发表时间：
2016-02-15
期刊：
影响因子：
5.7
通讯作者：
Tanter, Mickael
中科院分区：
文献类型：
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作者：
Demene, Charlie;Tiran, Elodie;Tanter, Mickael
应用超快多普勒断层成像技术（UFD-T）对啮齿动物脑血流动力学进行了4D超声微血管成像。使用超声波平面波传输以非常高的帧速率（每秒18，000帧）对大鼠大脑进行体内实时成像。这种超快的帧速率允许对血管进行远远超出常规超声检查的高灵敏度和宽视场的2D多普勒成像。体素各向异性（100 μ m × 100 μ m × 500 μ m）进行了校正，通过使用断层扫描的方法，其中包括超快采集重复不同的成像平面方向在多个心动周期。UFT-D允许对深层血管（最大20 mm）进行4D动态微血管成像，具有非常高的4D分辨率（分别为100 μ m x 100 μ m x 100 μ m和10 ms）和对全脑成像技术中小血管（>1 mm/s）流动的高灵敏度，无需任何造影剂。体内4D超声微血管成像可能成为研究脑血流动力学的有价值的工具，例如缺血性卒中恢复后的脑血流自动调节或血管重塑，以及更普遍地，肿瘤血管对治疗性治疗的反应。(C)2015 Elsevier Inc. All rights reserved.
4D ultrasound microvascular imaging was demonstrated by applying ultrafast Doppler tomography (UFD-T) to the imaging of brain hemodynamics in rodents. In vivo real-time imaging of the rat brain was performed using ultrasonic plane wave transmissions at very high frame rates (18,000 frames per second). Such ultrafast frame rates allow for highly sensitive and wide-field-of-view 2D Doppler imaging of blood vessels far beyond conventional ultrasonography. Voxel anisotropy (100 mu m x 100 mu m x 500 mu m) was corrected for by using a tomographic approach, which consisted of ultrafast acquisitions repeated for different imaging plane orientations over multiple cardiac cycles. UFT-D allows for 4D dynamic microvascular imaging of deep-seated vasculature (up to 20 mm) with a very high 4D resolution (respectively 100 mu m x 100 mu m x 100 mu m and 10 ms) and high sensitivity to flow in small vessels (>1 mm/s) for a whole-brain imaging technique without requiring any contrast agent. 4D ultrasound microvascular imaging in vivo could become a valuable tool for the study of brain hemodynamics, such as cerebral flow autoregulation or vascular remodeling after ischemic stroke recovery, and, more generally, tumor vasculature response to therapeutic treatment. (C) 2015 Elsevier Inc. All rights reserved.