In vivo cancer targeting and imaging with semiconductor quantum dots
In vivo cancer targeting and imaging with semiconductor quantum dots
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DOI：
10.1038/nbt994
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发表时间：
2004-08-01
影响因子：
46.9
通讯作者：
Nie, SM
中科院分区：
文献类型：
--
作者：
Gao, XH;Cui, YY;Nie, SM
我们描述了基于半导体量子点（QDs）的多功能纳米粒子探针的发展，用于活体动物的癌症靶向和成像。结构设计包括用ABC三嵌段共聚物封装发光量子点，并将这种两亲性聚合物连接到肿瘤靶向配体和药物传递功能上。在裸鼠中生长的人前列腺癌的体内靶向研究表明，QD探针通过增强肿瘤部位的渗透性和滞留性以及与癌症特异性细胞表面生物标志物的抗体结合在肿瘤中积累。通过皮下注射QD标记的癌细胞和全身注射多功能QD探针，我们在体内条件下实现了癌细胞的敏感和多色荧光成像。我们还集成了一个全身宏观照明系统与波长分辨光谱成像，以有效地去除背景和精确描绘弱光谱特征。这些结果为体内分子靶点的超灵敏和多路成像提供了新的可能性。
We describe the development of multifunctional nanoparticle probes based on semiconductor quantum dots (QDs) for cancer targeting and imaging in living animals. The structural design involves encapsulating luminescent QDs with an ABC triblock copolymer and linking this amphiphilic polymer to tumor-targeting ligands and drug-delivery functionalities. In vivo targeting studies of human prostate cancer growing in nude mice indicate that the QD probes accumulate at tumors both by the enhanced permeability and retention of tumor sites and by antibody binding to cancer-specific cell surface biomarkers. Using both subcutaneous injection of QD-tagged cancer cells and systemic injection of multifunctional QD probes, we have achieved sensitive and multicolor fluorescence imaging of cancer cells under in vivo conditions. We have also integrated a whole-body macro-illumination system with wavelength-resolved spectral imaging for efficient background removal and precise delineation of weak spectral signatures. These results raise new possibilities for ultrasensitive and multiplexed imaging of molecular targets in vivo.