Probing ligand-protein recognition with sum-frequency generation spectroscopy:: The avidin-biocytin case
Probing ligand-protein recognition with sum-frequency generation spectroscopy:: The avidin-biocytin case
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DOI：
10.1002/cphc.200400213
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发表时间：
2004-11-12
期刊：
影响因子：
2.9
通讯作者：
Peremans, A
中科院分区：
文献类型：
--
作者：
Dreesen, L;Sartenaer, Y;Peremans, A
红外/可见和频产生（SFG）光谱用于研究一种衍生的维生素（生物胞素）吸附在氟化钙底物上对蛋白质（亲和素）的识别。该过程的特异性是通过用牛血清白蛋白或饱和亲和素代替亲和素来测试的。SFG光谱显示，只有当生物细胞素薄膜暴露于亲和素时，CH和NH光谱范围才会发生剧烈的变化。将SFG数据与傅里叶变换红外反射吸收光谱（FT-IRRAS）在某些光谱范围内的比较，说明了非线性光谱在研究和检测生物分子间识别方面的优势。
Infrared/visible sum-frequency generation (SFG) spectroscopy is used to study the recognition of a protein (avidin) by a derived vitamin (biocytin) adsorbed on a calcium fluoride substrate. The specificity of the process is tested by replacing avidin with bovine serum albumin or presaturated avidin. The SFG spectroscopy shows drastic modifications in the CH and NH spectral ranges only upon exposure of the biocytin film to avidin. The comparison of the SFG data with Fourier transform infrared reflection absorption spectra (FT-IRRAS) in the some spectral ranges illustrates the advantages of nonlinear spectroscopy for studying and detecting recognition between biomolecules.