The Resistome of Pseudomonas aeruginosa in Relationship to Phenotypic Susceptibility
The Resistome of Pseudomonas aeruginosa in Relationship to Phenotypic Susceptibility
复制标题
DOI：
10.1128/aac.03954-14
复制
发表时间：
2015-01-01
影响因子：
4.9
通讯作者：
Gardner, Humphrey
中科院分区：
文献类型：
--
作者：
Kos, Veronica N.;Deraspe, Maxime;Gardner, Humphrey
铜绿假单胞菌的许多临床分离株由于其对多种抗生素的耐药性而引起难以根除的感染。通过2003年至2012年期间从不同地理位置收集的390株铜绿假单胞菌临床分离株的基因组序列确定了耐药的关键遗传决定因素，并与美罗培南、左氧氟沙星和阿米卡星的微生物敏感性数据相关。β-内酰胺酶和整合子盒排列在已建立的序列类型ST 111（主要是O 12）和ST 235（O 11）的多药耐药谱系中富集。这项研究证明了下一代测序（NGS）在定义相关耐药元件方面的实用性，并强调了铜绿假单胞菌内耐药决定因素的多样性。该信息在进一步设计用于治疗铜绿假单胞菌感染的诊断和治疗方法中是有价值的。
Many clinical isolates of Pseudomonas aeruginosa cause infections that are difficult to eradicate due to their resistance to a wide variety of antibiotics. Key genetic determinants of resistance were identified through genome sequences of 390 clinical isolates of P. aeruginosa, obtained from diverse geographic locations collected between 2003 and 2012 and were related to microbiological susceptibility data for meropenem, levofloxacin, and amikacin. beta-Lactamases and integron cassette arrangements were enriched in the established multidrug-resistant lineages of sequence types ST111 (predominantly O12) and ST235 (O11). This study demonstrates the utility of next-generation sequencing (NGS) in defining relevant resistance elements and highlights the diversity of resistance determinants within P. aeruginosa. This information is valuable in furthering the design of diagnostics and therapeutics for the treatment of P. aeruginosa infections.