Temporal profiles of nuclear receptor gene expression reveal coordinate transcriptional responses during Drosophila development
Temporal profiles of nuclear receptor gene expression reveal coordinate transcriptional responses during Drosophila development
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DOI：
10.1210/me.2002-0430
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发表时间：
2003-11-01
影响因子：
--
通讯作者：
Thummel, CS
中科院分区：
文献类型：
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作者：
Sullivan, AA;Thummel, CS
最近完成的果蝇基因组序列揭示了核受体超家族的21个成员。这些基因中的许多基因在转录上受到类固醇激素蜕皮激素的调节，并在变态的发生过程中发挥作用，包括EcR/USP蜕皮激素受体异二聚体。作为对这个基因家族的基因组分析的第一步，我们已经表征了所有可检测到的核受体转录本在整个生命周期中主要蜕皮激素调节的发育过渡的表达模式：胚胎发生，幼虫蜕皮，蛹形成，和预蛹过渡。我们发现一个意想不到的密切的时间关系DHR 3，E75 B，β FTZ-F1表达后，每个主要的蜕皮激素脉冲检查，反映了已知的交叉调节这些基因在包皮的相互作用，并表明它们在生命周期的其他阶段一起行动。此外，E75 A、E78 B和DHR 4以与DHR 3、E75 B和betaFTZ-F1可重复的方式表达，表明它们与该调节级联交叉。最后，我们发现，已知的蜕皮激素诱导的初级反应转录协调诱导的时候，蜕皮类固醇滴度低，这意味着存在新的，尚未表征，时间信号在果蝇。
The recent completion of the Drosophila genome sequence revealed 21 members of the nuclear receptor superfamily. Many of these genes are transcriptionally regulated by the steroid hormone ecdysone and play a role during the onset of metamorphosis, including the EcR/USP ecdysone receptor heterodimer. As a first step toward a genomic analysis of this gene family, we have characterized the temporal patterns of expression for all detectable nuclear receptor transcripts throughout major ecdysone-regulated developmental transitions in the life cycle: embryogenesis, a larval molt, puparium formation, and the prepupal-pupal transition. We find an unexpected close temporal relationship between DHR3, E75B, and betaFTZ-F1 expression after each major ecdysone pulse examined, reflecting the known cross-regulatory interactions of these genes in prepupae and suggesting that they act together at other stages in the life cycle. In addition, E75A, E78B, and DHR4 are expressed in a reproducible manner with DHR3, E75B, and betaFTZ-F1, suggesting that they intersect with this regulatory cascade. Finally, we find that known ecdysone-inducible primary-response transcripts are coordinately induced at times when the ecdysteroid titer is low, implying the existence of novel, as yet uncharacterized, temporal signals in Drosophila.