Phenylpropanoid amides from Alisma orientalis and their protective effects against H2O2-induced damage in SH-SY5Y cells
Phenylpropanoid amides from Alisma orientalis and their protective effects against H2O2-induced damage in SH-SY5Y cells
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DOI：
10.1016/j.phytol.2017.05.027
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发表时间：
2017-09
影响因子：
1.7
通讯作者：
Xiulan Xin;Zhen-long Yu;Xiangge Tian;Jiangchun Wei;Chao Wang;Xiaokui Huo;Jing Ning;Lei Feng;
中科院分区：
文献类型：
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作者：
Xiulan Xin;Zhen-long Yu;Xiangge Tian;Jiangchun Wei;Chao Wang;Xiaokui Huo;Jing Ning;Lei Feng;
从泽泻（Alisma orientalistogether）中分离得到5个新的苯丙酰胺类化合物：N-反式阿魏酰-N ′-顺式阿魏酰-尸胺（1），N，N′-反式二阿魏酰-3-氧代尸胺（2），N-反式阿魏酰-N ′-顺式阿魏酰-3-羟基尸胺（3），N，N′-顺式二阿魏酰-3-羟基尸胺（4），N-反式对香豆酰-N′-反式阿魏酰-3-羟基尸胺（5）。通过1D和2D NMR和HRESIMS光谱分析进行了结构鉴定。以人多巴胺能神经母细胞瘤细胞（SH-SY 5 Y）为模型，研究了化合物对HCE-2的抑制活性、抗氧化活性及对H2 O2诱导的细胞损伤的保护作用。化合物3、6和7显示出中等的抗氧化活性，IC 50值在36.9-40.7 μM之间。化合物5表现出明显的保护活性，而化合物1、2、4、7和8表现出中等的保护活性。
Five new phenylpropanoid amides, includingN-trans-feruloyl-N′-cis-feruloyl-cadaverine (1),N,N′-trans-diferuloyl-3-oxo-cadaverine (2),N-trans-feruloyl-N′-cis-feruloyl-3-hydroxy-cadaverine (3),N,N′-cis-diferuloyl-3-hydroxy-cadaverine (4),N-trans-p-coumaroyl-N′-trans-feruloyl-3-hydroxy-cadaverine (5), were isolated fromAlisma orientalistogether with four known analogues. Their structural elucidations were conducted by using 1D and 2D NMR and HRESIMS spectroscopic analyses. The isolated compounds were assayed for their inhibitory activities against HCE-2, anti-oxidant effects, and their protective effects on H2O2-induced damage in human dopaminergic neuroblastoma cells (SH-SY5Y). Compounds3,6, and7displayed moderate anti-oxidant activities with IC50values in the range of 36.9–40.7 μM. Compound5showed significant protective activity, while compounds1,2,4,7, and8showed moderate protective activities.