Dynamic dual-crosslinking antibacterial hydrogel with enhanced bio-adhesion and self-healing activities for rapid hemostasis in vitro and in vivo
Dynamic dual-crosslinking antibacterial hydrogel with enhanced bio-adhesion and self-healing activities for rapid hemostasis in vitro and in vivo
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DOI：
10.1016/j.matdes.2023.112244
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发表时间：
2023-08
期刊：
影响因子：
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通讯作者：
Ranran Si;Y. Wang;Yuchun Yang;A. Javeed;Jianing Chen;Bingnan Han
中科院分区：
文献类型：
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作者：
Ranran Si;Y. Wang;Yuchun Yang;A. Javeed;Jianing Chen;Bingnan Han
基于天然高分子丝素蛋白（SF）的生物粘合剂因其良好的生物相容性和生物可降解性，在快速止血剂的开发中备受关注。但由于其生物活性、力学性能和生物粘附性能的不足，限制了其在生物医学领域的应用。本文基于动态双交联机理，将单宁酸包覆丝微纤维（TA@SMF）基序引入聚乙烯醇（PB）水凝胶网络中，制备了一种基于丝微纤维（SMFs）的多功能止血水凝胶。内聚增强策略，沿着动态硼酸酯-二醇键和氢键，协同地为水凝胶提供增强的生物粘附性和自愈合性质。TA@SMF/PB凝胶的断裂伸长率大于600%，在水中浸泡24 h后仍能粘附于猪皮上，体外实验表明TA@SMF/PB凝胶具有良好的抗菌、抗氧化、生物相容性和止血性能，体内降解和小鼠肝脏止血试验进一步验证了其生物安全性和快速止血性能。TA@SMF-0.5组的止血时间为42.0 ± 4.9 s，显著短于其他实验组。这项研究是用TA作为止血材料功能化SMF的原始报告，它提供了一个多功能的基于SMF的工具包，为快速止血和感染性伤口愈合提供了有前途的候选材料。
Bio-adhesives based on natural polymer silk fibroin (SF) are high-profiled in the development of rapid hemostatic agents because of their good biocompatibility and biodegradability. However, the lack of bioactivity, mechanical and bio-adhesion performance, restrict their use in the biomedical field. Herein, based on the dynamic dual-crosslinking mechanism, we fabricated a silk microfibers (SMFs)-based multifunctional hemostatic hydrogel by incorporating tannic acid-coated SMF (TA@SMF) motifs into the network of poly(vinyl alcohol)-borax (PB) hydrogel. The cohesion-enhancing strategy, along with dynamic borate-diol bonds and hydrogen bonds, synergically provide the hydrogel with enhanced bio-adhesion and self-healing properties. TA@SMF/PB gel has an elongation at break of more than 600% and adheres to pig skin even after 24 h immersion in water.In vitroexperiments have shown good antibacterial, antioxidant, biocompatibility, and hemostatic properties of the TA@SMF/PB gel.In vivodegradation and mouse liver hemostatic test further verified its biosafety and rapid hemostasis performance. The hemostatic time of 42.0 ± 4.9 s in TA@SMF-0.5 was significantly shorter than that in other experimental groups. This study is the original report for functionalizing SMFs with TA as a hemostasis material, which affords a versatile SMF-based toolkit that provides promising candidate materials for rapid hemostatic and infectious wound healing.