CircSEC24A upregulates TGFBR2 expression to accelerate pancreatic cancer proliferation and migration via sponging to miR-606.
CircSEC24A upregulates TGFBR2 expression to accelerate pancreatic cancer proliferation and migration via sponging to miR-606.
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CircSEC24A 通过海绵作用上调 TGFBR2 表达，加速胰腺癌增殖和迁移 miR-606
DOI：
10.1186/s12935-021-02392-y
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发表时间：
2021-12-14
影响因子：
5.8
通讯作者：
Jiang J
中科院分区：
文献类型：
--
作者：
Chen Y;Xu S;Liu X;Jiang X;Jiang J
研究背景环状RNA（Circular RNA，circRNA）是一种新型的非编码RNA，通过特殊的选择性剪接产生，广泛表达于真核细胞的细胞质中。它在包括癌症在内的各种疾病中发挥不同的作用，并发挥不同的功能。然而，目前关于circRNA在胰腺癌中的特异性功能的报道还很少。方法采用实时荧光定量PCR方法检测circSEC 24 A在胰腺癌组织和细胞系中的表达水平。此外，我们还通过EDU和Transwell等功能实验，探讨circSEC 24 A对胰腺癌细胞增殖和侵袭能力的影响。最后，通过双荧光素酶报告基因等机制研究，探讨circSEC 24 A、miR-606和TGF-β R 2之间的对应关系。此外，敲低circSEC 24 A基因可显著抑制胰腺癌细胞的增殖、迁移和侵袭能力，而miR-606抑制剂则可明显抵消这些作用。结论circSEC 24 A通过影响miR-606/TGFBR 2轴，进而影响胰腺癌的发生发展。因此，circSEC 24 A可能是影响胰腺癌早期诊断和预后的重要生物标志物。
BackgroundCircular RNA (circRNA), producing by special selective splicing, was widely expressed in the cytoplasm of eukaryotic cells as a newly non-coding RNAs. It played different roles in a variety of diseases including cancer and performed different functions. Nonetheless, reports on the specific function of circRNA in pancreatic cancer (PC) were still rarely so far. In particular, the role of circSEC24A in PC remains unclear.MethodsReal-time fluorescent quantitative PCR was used to evaluate the expression level of circSEC24A in pancreatic cancer tissues and cell lines. Furthermore, we used some functional experiments, such as EDU and Transwell assays, to explore the effects of circSEC24A on the proliferation and invasiveness of pancreatic cancer. Finally, the corresponding relationship among circSEC24A, miR-606 and TGFBR2 was explored by dual luciferase reporter and other mechanism studies.ResultsThe expression of circSEC24A in both pancreatic cancer tissues and cell lines was evidently up-regulated. Furthermore, knockdown of circSEC24A significantly inhibited the proliferative, migration and invasive capacity of pancreatic cancer cells, whereas miR-606 inhibitor obviously counteracted these effects. Further study confirmed that circSEC24A alleviated suppression on target TGFBR2 expression by directly sponging miR-606 and then influenced the tumorigenesis of pancreatic cancer.ConclusionsThese findings indicated that the progression of pancreatic cancer can be driven by circSEC24A influencing miR-606/TGFBR2 axis. Therefore, circSEC24A might be used as a critical biomarker influencing the early diagnosis and prognosis of pancreatic cancer.
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DOI：
10.1152/ajpgi.00118.2020
发表时间：
2020-12-01
影响因子：
4.5
作者：
Zhang, Xiutian;Tan, Peng;Du, Lei
通讯作者：
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影响因子：
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Zhang, Meng
影响因子：
5.8
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Xiong X;Feng J;Yang X;Li H;Shi Q;Tao J;Chang J
通讯作者：
Chang J
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影响因子：
37.3
作者：
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