Organogenesis and Somatic Embryogenesis from Young Flower Buds of Agapanthus africanus Hoffmanns
Organogenesis and Somatic Embryogenesis from Young Flower Buds of Agapanthus africanus Hoffmanns
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DOI：
10.5511/plantbiotechnology.14.23
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发表时间：
1997-04
影响因子：
1.6
通讯作者：
K. Supaibulwatana;M. Mii
中科院分区：
文献类型：
--
作者：
K. Supaibulwatana;M. Mii
以百子莲幼蕾为外植体，在MS +30 g·L-1蔗糖+2g·L-1结冷胶+1mg·L-1激动素或噻苯隆+1mg·L-1 NAA的培养基上直接诱导不定芽和根。在含有NAA、毒莠定或2，4-D的培养基上，以低浓度的毒莠定或2，4-D效果最好，诱导出的愈伤组织疏松，植株再生率高。将愈伤组织转移到不含植物生长调节剂或含有1 mg·L-1玉米素的MS培养基上，可诱导出芽。在附加2，4-D或毒莠定的培养基上，疏松的愈伤组织也能形成致密的瘤状愈伤组织。1 mg·L-1毒莠定诱导的致密愈伤组织发育为体细胞胚。在芽和胚再生体系中，当它们转移到不含植物生长调节剂的培养基上时，获得完整的植株。
Adventitious shoots and roots were directly formed from young flower bud explants of Agapanthus africanus on Murashige and Skoog (MS) medium containing 30g·L-1 sucrose, 2g·L-1 gellan gum, and kinetin or thidiazuron at 1mg·L-1 in combination with 1mg·L-1 NAA. Friable calli with a high rate of plant regeneration were also induced by culturing the explants on medium containing NAA, picloram or 2, 4-D at concentrations varying from 1 to 10mg·L-1, especially at low concentrations of picloram or 2, 4-D. Shoots were readily induced from these calli when transferred onto plant growth regulator-free or 1mg·L-1 zeatin-containing MS medium. Nodular compact calli were also formed with friable calli on medium supplemented with 2, 4-D or picloram. The compact calli induced with 1mg·L-1 picloram developed into somatic embryos. In both shoot and embryo regeneration systems, complete plantlets were obtained when they were transferred onto a medium containing no plant growth regulator.