Fusarium virguliforme Transcriptional Plasticity Is Revealed by Host Colonization of Maize versus Soybean
Fusarium virguliforme Transcriptional Plasticity Is Revealed by Host Colonization of Maize versus Soybean
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DOI：
10.1105/tpc.19.00697
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发表时间：
2020-02-01
期刊：
影响因子：
11.6
通讯作者：
Day, Brad
中科院分区：
文献类型：
--
作者：
Baetsen-Young, Amy;Wai, Ching Man;Day, Brad
对镰刀菌定殖的转录组分析揭示了感染程序的广泛重新布线，分别导致玉米和大豆出现无症状和有症状的表型。我们利用 Fusarium virguliforme 的广泛宿主范围来识别在玉米 (Zea mays) 和大豆 (Glycine max) 定殖期间分别导致内生或致病生活方式的差异真菌反应。为了提供调查转录组景观的基础，我们使用 PacBio 测序对 F. virguliforme 进行了改进的从头基因组组装和注释。接下来，我们对有症状宿主大豆和无症状宿主玉米进行了 F. virguliforme 定植和感染的高分辨率时间过程。比较转录组分析发现，网络几乎完全重新布线，在定植不同植物宿主时，平均基因共表达模块重叠率低于 8%。源自宿主特定时间诱导基因的转录组的分歧是感染和定植的核心，包括碳水化合物活性酶（CAZymes）和坏死诱导效应子。接种后第 2 天，大豆中独特地诱导了 Zn(II)-Cys6 转录因子的上调，表明病原体对大豆的毒力增强。总的来说，本文描述的数据表明 F. virguliforme 通过转录可塑性调节不同的感染特征。
Transcriptomic analysis of Fusarium virguliforme colonization revealed extensive rewiring of infection programs, leading to asymptomatic and symptomatic phenotypes in maize and soybean, respectively. We exploited the broad host range of Fusarium virguliforme to identify differential fungal responses leading to either an endophytic or a pathogenic lifestyle during colonization of maize (Zea mays) and soybean (Glycine max), respectively. To provide a foundation to survey the transcriptomic landscape, we produced an improved de novo genome assembly and annotation of F. virguliforme using PacBio sequencing. Next, we conducted a high-resolution time course of F. virguliforme colonization and infection of both soybean, a symptomatic host, and maize, an asymptomatic host. Comparative transcriptomic analyses uncovered a nearly complete network rewiring, with less than 8% average gene coexpression module overlap upon colonizing the different plant hosts. Divergence of transcriptomes originating from host specific temporal induction genes is central to infection and colonization, including carbohydrate-active enzymes (CAZymes) and necrosis inducing effectors. Upregulation of Zn(II)-Cys6 transcription factors were uniquely induced in soybean at 2 d postinoculation, suggestive of enhanced pathogen virulence on soybean. In total, the data described herein suggest that F. virguliforme modulates divergent infection profiles through transcriptional plasticity.