Faster high-speed atomic force microscopy for imaging of biomolecular processes
Faster high-speed atomic force microscopy for imaging of biomolecular processes
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DOI：
10.1063/5.0032948
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发表时间：
2021-03-01
影响因子：
1.6
通讯作者：
Ando, Toshio
中科院分区：
文献类型：
--
作者：
Fukuda, Shingo;Ando, Toshio
高速原子力显微镜（HS-AFM）能够以每秒1-12.5帧（fps）的速率观察蛋白质分子的功能活动，这取决于成像条件，样品高度和脆性。为了满足可观察动态分子过程不断扩大的需求，迫切需要更快、干扰更少的HS-AFM。然而，即使速度性能提高50%也会带来巨大的挑战，因为主要限速组件的快速响应优化已接近成熟。本文提出了一种替代方法，可以降低反馈控制误差，从而提高成像速率。这种方法可以通过对软件和硬件的微小修改在任何HS-AFM系统中实现。由此产生的更快，更少的干扰成像能力证明了相对脆弱的肌动蛋白丝和微管附近的视频速率，和肌动蛋白聚合，发生通过弱分子间相互作用，在类似的8 fps的成像。(C)2021年作者。
High-speed atomic force microscopy (HS-AFM) has enabled observing protein molecules during their functional activity at rates of 1-12.5 frames per second (fps), depending on the imaging conditions, sample height, and fragility. To meet the increasing demand for the great expansion of observable dynamic molecular processes, faster HS-AFM with less disturbance is imperatively needed. However, even a 50% improvement in the speed performance imposes tremendous challenges, as the optimization of major rate-limiting components for their fast response is nearly matured. This paper proposes an alternative method that can lower the feedback control error and thereby enhance the imaging rate. This method can be implemented in any HS-AFM system by minor modifications of the software and hardware. The resulting faster and less-disturbing imaging capabilities are demonstrated by the imaging of relatively fragile actin filaments and microtubules near the video rate, and of actin polymerization that occurs through weak intermolecular interactions, at similar to 8 fps. (C) 2021 Author(s).