Fabrication and characterization of DNA-functionalized GaN nanowires
Fabrication and characterization of DNA-functionalized GaN nanowires
复制标题
DNA功能化GaN纳米线的制备和表征
DOI：
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发表时间：
2007
期刊：
影响因子：
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通讯作者：
P. Pehrsson
中科院分区：
文献类型：
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作者：
B. Simpkins;K. McCoy;L. Whitman;P. Pehrsson
平面GaN衬底和单个GaN纳米线都已经用单链DNA功能化，这是使用GaN器件进行无标记生物传感的重要过程。如XPS所证实的，在羟基化GaN上沉积功能性氨丙基三甲氧基硅烷层。然后将双官能戊二醛层偶联到硅烷上，呈现羰基，用于随后与胺封端的荧光标记DNA的缩合反应。原子力和扫描电子显微镜的研究表明，功能层是光滑的，均匀沉积，并在两个平面表面和单个纳米线上至少20 nm厚。氧等离子体处理与硅酮掩模结合使用以图案化官能化表面和纳米线。DNA保持固定在纳米线后，通过超声波处理和介电电泳对齐释放到悬浮液中，证明了这种功能化化学的潜力，为“自下而上”的制造和自组装的纳米线传感器阵列。
Both planar GaN substrates and individual GaN nanowires have been functionalized with single-stranded DNA, an important process for use of GaN devices for label-free biosensing. A functional aminopropyltrimethoxysilane layer was deposited on hydroxylated GaN, as confirmed by XPS. A bifunctional glutaraldehyde layer was then coupled to the silane, presenting carbonyl groups for subsequent condensation reaction with amine-terminated, fluorescently labeled DNA. Atomic force and scanning electron microscopy studies indicated the functional layers are smooth, uniformly deposited, and at least 20 nm thick on both the planar surfaces and on individual nanowires. An oxygen plasma treatment was used in conjunction with a silicone mask to pattern the functionalized surfaces and nanowires. DNA remained immobilized on the nanowires following their release into suspension via sonication and dielectrophoretic alignment, demonstrating the potential of this functionalization chemistry for ‘bottom-up’ fabrication and self-assembly of nanowire sensor arrays.