Phasing of muscle gene expression with fasting-induced recovery growth in Atlantic salmon.
Phasing of muscle gene expression with fasting-induced recovery growth in Atlantic salmon.
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DOI：
10.1186/1742-9994-6-18
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发表时间：
2009-08-24
影响因子：
2.8
通讯作者：
Johnston IA
中科院分区：
文献类型：
--
作者：
Bower NI;Taylor RG;Johnston IA
许多鱼类在自然界中经历长时间的禁食，通常与水温和猎物可用性或产卵迁移的季节性降低有关。在营养限制期间，新陈代谢发生变化，通过分解代谢过程提供细胞能量。肌肉尤其受到长时间禁食的影响，因为肌原纤维蛋白是主要的能量来源。研究禁食和再投喂对大西洋鲑（萨尔莫salar L.）我们使用qPCR分析了参与肌生成、生长信号传导、脂质生物合成和肌原纤维蛋白降解和合成途径的基因的表达。基因表达数据的层次聚类揭示了三个聚类。第一个簇包括参与脂质代谢和三酰甘油合成的基因（ALDOB、DGAT 1和LPL），其在再饲养后3- 14天表达达到峰值。第二个簇包括ADIPOQ、MLC 2、IGF-I和TALDO 1，在再饲养后14- 32天表达高峰。簇III包含强烈下调的基因作为对进食的初始反应，包括泛素连接酶MuRF 1和MAFbx，生肌调节因子和一些代谢基因。禁食鲑鱼对再喂食的早期反应包括三酰甘油的合成和脂肪形成分化程序的激活。MuRF 1和MAFbx的抑制分别可能导致肌原纤维蛋白的降解减少和伴随的产生增加。这两个过程之前的任何增加的表达肌调节因子和IGF-I。这些反应可能是一个必要的策略，动物适应长期的食物匮乏，从而补充能量储备之前恢复肌生成。
Many fish species experience long periods of fasting in nature often associated with seasonal reductions in water temperature and prey availability or spawning migrations. During periods of nutrient restriction, changes in metabolism occur to provide cellular energy via catabolic processes. Muscle is particularly affected by prolonged fasting as myofibrillar proteins act as a major energy source. To investigate the mechanisms of metabolic reorganisation with fasting and refeeding in a saltwater stage of Atlantic salmon (Salmo salar L.) we analysed the expression of genes involved in myogenesis, growth signalling, lipid biosynthesis and myofibrillar protein degradation and synthesis pathways using qPCR. Hierarchical clustering of gene expression data revealed three clusters. The first cluster comprised genes involved in lipid metabolism and triacylglycerol synthesis (ALDOB, DGAT1 and LPL) which had peak expression 3-14d after refeeding. The second cluster comprised ADIPOQ, MLC2, IGF-I and TALDO1, with peak expression 14-32d after refeeding. Cluster III contained genes strongly down regulated as an initial response to feeding and included the ubiquitin ligases MuRF1 and MAFbx, myogenic regulatory factors and some metabolic genes. Early responses to refeeding in fasted salmon included the synthesis of triacylglycerols and activation of the adipogenic differentiation program. Inhibition of MuRF1 and MAFbx respectively may result in decreased degradation and concomitant increased production of myofibrillar proteins. Both of these processes preceded any increase in expression of myogenic regulatory factors and IGF-I. These responses could be a necessary strategy for an animal adapted to long periods of food deprivation whereby energy reserves are replenished prior to the resumption of myogenesis.
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影响因子：
2.8
作者：
Bower, Neil I.;Li, Xuejun;Johnston, Ian A.
通讯作者：
Johnston, Ian A.
影响因子：
4
作者：
Gabillard, Jean-Charles;Kamangar, Barzan Bahrami;Montserrat, Nuria
通讯作者：
Montserrat, Nuria
影响因子：
4.8
作者：
Banki, K;Hutter, E;Perl, A
通讯作者：
Perl, A
影响因子：
6.5
作者：
Fu, YC;Luo, NL;Garvey, WT
通讯作者：
Garvey, WT
影响因子：
4.8
作者：
Artaza, JN;Bhasin, S;Gonzalez-Cadavid, NF
通讯作者：
Gonzalez-Cadavid, NF