A colorimetric boronic acid based sensing ensemble for carboxy and phospho sugars.
A colorimetric boronic acid based sensing ensemble for carboxy and phospho sugars.
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DOI：
10.1021/ol060279
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发表时间：
2006-03
期刊：
影响因子：
5.2
通讯作者：
Tianzhi Zhang;E. Anslyn
中科院分区：
文献类型：
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作者：
Tianzhi Zhang;E. Anslyn
[结构：合成了一种以镉为中心的三硼酸受体，并测定了它与各种阴离子糖的结合特性。该受体对不同的阴离子糖表现出高亲和力，尤其是葡萄糖酸，其在中性pH值下的结合常数接近约10（7）M（-）（1）。此外，使用指示剂置换试验，在添加阴离子糖后观察到邻苯二酚紫的颜色变化。该比色试验用作定性分析客体亲和力的简易筛选技术。
[structure: see text] A cadmium-centered tris-boronic acid receptor was synthesized, and its binding properties toward various anionic sugars were determined. This receptor shows high affinity for different anionic sugars, especially gluconic acid, which has an association constant near approximately 10(7) M(-)(1) at neutral pH. Further, using an indicator displacement assay, a color change of pyrocatechol violet was observed upon addition of anionic sugars. This colorimetric test was used as a facile screening technique to qualitatively analyze guest affinities.