IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : I. ESTIMATION OF A AND B ISOANTIBODIES IN HUMAN SERUM BY THE QUANTITATIVE PRECIPITIN METHOD.
IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : I. ESTIMATION OF A AND B ISOANTIBODIES IN HUMAN SERUM BY THE QUANTITATIVE PRECIPITIN METHOD.
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DOI：
10.1084/jem.82.3.207
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发表时间：
1945-09-01
期刊：
影响因子：
--
通讯作者：
Bezer AE
中科院分区：
文献类型：
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作者：
Kabat EA;Bezer AE
1.用纯化的A、B型血，在含有同源异凝素的血清中，可获得猪胃粘液中的特异性沉淀素反应物质。通过微量沉淀技术，可以按重量估算正常和免疫异凝素血清中抗-A和抗-B的含量。2.A类物质从O、B群血清中沉淀出的抗体N与A类血型物质免疫前后的A红细胞凝集素效价之间存在普遍的相关性。3.免疫个体血清中A物质与其同源异凝素的反应与其他抗原-抗体系统中的反应类型相同。
1. With purified blood group A and B specific substances from the gastric mucin of pigs precipitin reactions can be obtained in sera containing homologous isoagglutinins. By microquantitative precipitin technics the anti-A and anti-B content of normal and immune isoagglutinin-containing sera can be estimated on a weight basis. 2. There was a general correlation between antibody N precipitable from the sera of groups O and B by A substance and the agglutinin titer for A erythrocytes before and after immunization with blood group substances. 3. The reaction between A substance and its homologous isoagglutinin present in the sera of immunized individuals was of the same type as in other antigen-antibody systems.