Soluble antibody affinity chromatography technique investigated with ultratrace [125I]thyroxine.
Soluble antibody affinity chromatography technique investigated with ultratrace [125I]thyroxine.
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使用超痕量[125I]甲状腺素研究可溶性抗体亲和层析技术。
DOI：
10.1016/s0021-9673(01)97743-x
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Giese,RW
中科院分区：
文献类型：
--
作者：
Warden,BA;Giese,RW
将10 μ g量的[125I]甲状腺素进行DEAE琼脂糖层析，然后与抗体复合。在相同条件下，将[125 I]甲状腺素作为与抗体的复合物再循环通过第二个DEAE琼脂糖色谱柱，使[125 I]甲状腺素的保留发生变化，这可能构成从第一个色谱柱共洗脱干扰物的特定偏移。然后将[125 I]甲状腺素从抗体上解离，并置于第三个DEAE琼脂糖柱上，以进一步改变其色谱保留值。由于[125 I]甲状腺素的总回收率为36%，这种可溶性抗体亲和技术可能用于超痕量溶质（如甲状腺素）标记分析中的样品净化。
An amount of 10 pg of [125I]thyroxine was subjected to DEAE agarose chromatography and then complexed with an antibody. Recycling of the [125I]thyroxine now as a complex with the antibody through a second DEAE agarose column under the same conditions gave a change in the retention of the [125I]thyroxine that potentially constitutes a specific shift away from co-eluting interferences from the first column. The [125I]thyroxine was then dissociated from the antibody and subjected to a third DEAE agarose column for additional shifting of its chromatographic retention. Since the overall recovery of the [125I]thyroxine is 36%, this soluble antibody affinity technique potentially is useful for sample clean-up in labeling analysis of ultratrace solutes such as thryoxine.