Quantitative magnetization transfer imaging in postmortem multiple sclerosis brain.
Quantitative magnetization transfer imaging in postmortem multiple sclerosis brain.
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DOI：
10.1002/jmri.20984
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发表时间：
2007-07
影响因子：
4.4
通讯作者：
Miller, David H
中科院分区：
文献类型：
--
作者：
Schmierer, Klaus;Tozer, Daniel J;Scaravilli, Francesco;Altmann, Daniel R;Barker, Gareth J;Tofts, Paul S;Miller, David H
目的 研究多发性硬化症 (MS) 受试者死后大脑中髓磷脂含量、轴突密度和神经胶质增生与使用定量磁化转移 (qMT) MRI 获得的大分子质子 (fB) 分数和大分子池 (T2B) 的 T2 弛豫 (T2B) 的关系。 fB 和 T2B 是在 20 名 MS 受试者的未固定死后脑切片中获得的。髓磷脂含量、轴突计数和神经胶质增生的严重程度均通过组织学定量。使用 t 检验和多元回归进行分析。在未固定的死后 MS 大脑中获得的 MR 指数与文献报道的体内值一致。 Tr-髓磷脂（与髓磷脂含量成反比）与 1）fB（r = -0.80，P < 0.001）和 2）轴突计数（r = -0.79，P < 0.001）之间检测到显着相关性。 fB 在 1) 正常白质 (NAWM) 和髓鞘再生 WM 病变 (rWML) 之间存在差异（平均值：fB 6.9 [SD 2] 与 4.0 [1.8]，P = 0.01），以及 2) rWML 和脱髓鞘 WML（平均值：4.2 [2.2] 与 2.5 [1.3]，P = 0.016）。未检测到 T2B 与任何组织学测量之间存在关联。 MS WM 中的 fB 取决于髓磷脂含量，可能是监测患有这种疾病的患者的工具。
To investigate the relationship of myelin content, axonal density, and gliosis with the fraction of macromolecular protons (fB) and T2 relaxation of the macromolecular pool (T2B) acquired using quantitative magnetization transfer (qMT) MRI in postmortem brains of subjects with multiple sclerosis (MS). fB and T2B were acquired in unfixed postmortem brain slices of 20 subjects with MS. The myelin content, axonal count, and severity of gliosis were all quantified histologically. t-Tests and multiple regression were used for analysis. MR indices obtained in unfixed postmortem MS brains were consistent with in vivo values reported in the literature. A significant correlation was detected between Tr-myelin (inversely proportional to myelin content) and 1) fB (r = -0.80, P < 0.001) and 2) axonal count (r = -0.79, P < 0.001). fB differed between 1) normal-appearing white matter (NAWM) and remyelinated WM lesions (rWMLs) (mean: fB 6.9 [SD 2] vs. 4.0 [1.8], P = 0.01), and 2) rWMLs and demyelinated WMLs (mean: 4.2 [2.2] vs. 2.5 [1.3], P = 0.016). No association was detected between T2B and any of the histological measures. fB in MS WM is dependent on myelin content and may be a tool to monitor patients with this condition.