Drycells: Cell‐suspension micro liquid marbles for single‐cell picking
Drycells: Cell‐suspension micro liquid marbles for single‐cell picking
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Drycells：用于单细胞拾取的细胞悬浮微液体弹珠
DOI：
10.1002/adma.202300486
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发表时间：
2023
影响因子：
29.4
通讯作者：
Uto Koichiro
中科院分区：
文献类型：
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作者：
Tenjimbayashi Mizuki;Yamamoto Shota;Uto Koichiro
细胞采摘技术是细胞培养的关键技术。尽管最近开发的工具可以进行单细胞水平的挑选，但它们依赖于特殊技能或额外的设备。在这项工作中，报告了一种干粉，它用95%的水介质包裹单个到几个细胞，从而成为一种强大的细胞挑选工具。所提出的“干细胞”是通过将细胞悬浮液喷洒在疏水的气相二氧化硅纳米颗粒的粉床上而形成的。这些颗粒吸附在液滴表面，形成一个超疏水的外壳，防止干细胞合并。可以通过调整干细胞的大小和细胞悬浮液浓度来控制每个干细胞的微囊化细胞数量。此外，还有可能将一对正常或癌变的细胞包裹起来，并在单个干细胞内形成几个细胞克隆。筛分过程可以用来根据大小对干细胞进行分类。液滴的大小从一微米到数百微米不等。干燥细胞足够硬，可以用镊子收集；然而，离心法将它们分离成纳米颗粒和细胞悬浮层，分离出的颗粒是可回收的。可以使用各种处理技术，例如分裂聚结和内液体置换。相信所提出的干电池的应用将极大地提高单细胞分析的可及性和生产率。
Cell‐picking technology is essential for cell culturing. Although the recently developed tools enable single‐cell‐level picking, they rely on special skills or additional devices. In this work, a dry powder that encapsulates single to several cells with a >95% aqueous culture medium, thereby acting as a powerful cell‐picking tool, is reported. The proposed “drycells” are formed by spraying a cell suspension onto a powder bed of hydrophobic fumed silica nanoparticles. The particles adsorb to the droplet surface and form a superhydrophobic shell, which prevents the drycells from coalescence. The number of encapsulated cells per drycell can be controlled by adjusting the drycell size and cell‐suspension concentration. Moreover, it is possible to encapsulate a pair of normal or cancerous cells and create several cell colonies within a single drycell. A sieving process can be used to sort the drycells according to size. The size of the droplet can range from one to hundreds of micrometers. The drycells are sufficiently stiff to be collected using tweezers; however, centrifugation separates them into nanoparticles and cell‐suspension layers, with the separated particles being recyclable. Various handling techniques, such as splitting coalescence and inner liquid replacement, can be used. It is believed that the application of the proposed drycells will greatly improve the accessibility and productivity of single‐cell analysis.