Expression profile and function characterization of the MYB type transcription factor genes in wheat (Triticum aestivum L.) under phosphorus deprivation
Expression profile and function characterization of the MYB type transcription factor genes in wheat (Triticum aestivum L.) under phosphorus deprivation
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缺磷条件下小麦 MYB 型转录因子基因的表达谱和功能表征
DOI：
10.1007/s11738-015-1997-2
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发表时间：
2016-01-01
影响因子：
2.6
通讯作者：
Xiao, Kai
中科院分区：
文献类型：
--
作者：
Fang, Weibo;Ding, Weiwei;Xiao, Kai
MYB蛋白是植物中最大的转录因子家族之一，在调节植物对各种非生物胁迫的适应中起着关键作用。本研究对42个小麦MYB基因在缺磷胁迫下的表达谱和功能进行了分析。结果表明，小麦MYB基因在B中分别有21、11、5、2和1个同源。二穗草、水稻、玉米、大麦和拟南芥。系统发育分析表明，小麦MYB基因经历了5条进化途径。通过基因芯片分析，发现小麦MYB基因在正常条件下表现出不同的转录本，根据表达水平可分为高表达（8个成员）、中等表达（10个成员）、低表达（10个成员）和极低表达（14个成员）。在缺磷条件下，多数MYB基因的转录水平发生了显著改变，其中TaMYB 1、TaMYB 16和TaMYB 73;1表达上调，TaMYB 11、TaMYB 12、TaMYB 13 -2;1和TaMYB;6表达下调。TaMYB 1基因的转基因分析证实了它在介导植物对缺磷胁迫的耐受性中起重要作用。高表达TaMYB 1的转基因植株在缺磷胁迫下表现出较高的植株干重和磷积累量。总之，我们的研究表明，小麦MYB基因的一个子集涉及植物对Pi缺乏的反应，其中TaMYB 1在介导植物对Pi饥饿胁迫的耐受性中起着关键作用。
MYB proteins constitute one of the largest transcription factor families in plants and play critical roles in mediating plant adaptation to diverse abiotic stresses. In this study, fourty two of the MYB genes in wheat were subjected to the expression profile and function characterization analysis under phosphorus (Pi) deprivation. The results indicated that twenty-one, eleven, five, two, and one of the wheat MYB genes share homologous partners in B. Distachyon, rice, maize, barley, and Arabidopsis, respectively. Phylogenetic analysis suggested that the wheat MYB genes have evolved from five evolutionary pathways. Based on a microarray analysis, the wheat MYB genes were found to exhibit different transcripts under normal condition and can be grouped into various categories according to expression level, including those of high (eight members), moderate (ten members), low (ten members), and very low (fourteen members), respectively. Under Pi deprivation, most of the MYB genes showed significantly modified transcripts, in which TaMYB1, TaMYB16, and TaMYB73;1 were upregulated, and TaMYB11, TaMYB12, TaMYB13-2;1, and TaMYB;6 were downregulated. Transgenic analysis of TaMYB1, one of the upregulated genes, confirmed that it played an important role in mediating plant tolerance to Pi deprivation. The transgenic plants overexpressing TaMYB1 displayed higher plant drymass and more P accumulation than wild type under Pi deprivation. Together, our investigation indicates that a subset of wheat MYB genes involves plant response to Pi deficiency in which TaMYB1 plays critical roles in mediating plant tolerance to the Pi-starvation stress.