High density DNA loading on the M13 bacteriophage provides access to colorimetric and fluorescent protein microarray biosensors
High density DNA loading on the M13 bacteriophage provides access to colorimetric and fluorescent protein microarray biosensors
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DOI：
10.1039/c3cc38871a
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发表时间：
2013-01-01
影响因子：
4.9
通讯作者：
Cha, Jennifer N.
中科院分区：
文献类型：
--
作者：
Domaille, Dylan W.;Lee, Ju Hun;Cha, Jennifer N.
我们报道了M13噬菌体-DNA生物缀合物的合成、表征和蛋白质传感能力。DNA寡核苷酸通过酰腙键与M13偶联。在一种情况下，DNA酶在锚定到病毒外壳时保留了它们的催化能力，在另一项研究中，腙的动态性质允许在温和条件下从噬菌体中释放DNA。
We report the synthesis, characterization, and protein sensing capabilities of M13 bacteriophage-DNA bioconjugates. DNA oligonucleotides were conjugated to M13 through acyl hydrazone linkages. In one case, DNAzymes retained their catalytic ability when anchored to the virus coat, and in a separate study, the dynamic nature of the hydrazone allowed for liberation of DNA from the phage under mild conditions.