Platinum nanoparticles for the photothermal treatment of Neuro 2A cancer cells
Platinum nanoparticles for the photothermal treatment of Neuro 2A cancer cells
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DOI：
10.1016/j.biomaterials.2013.03.077
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发表时间：
2013-07-01
期刊：
影响因子：
14
通讯作者：
Wu, Hui-Fen
中科院分区：
文献类型：
--
作者：
Manikandan, M.;Hasan, Nazim;Wu, Hui-Fen
本研究证明了使用改进的方法在1-21 nm的窄尺寸范围内有效地合成了五种不同尺寸/形状的Pt NPs，并且通过使用光学显微镜观察，色氨酸蓝排斥试验，MIT测定和ICP-MS。与其他四种Pt NPs相比，尺寸为5-6 nm的Pt NPs-C显示出优越的上级非细胞毒性特性。这些非细胞毒性Pt NP用于使用近IR 1064 nm激光照射的Neuro 2A细胞系的成功光热处理。Pt NPs-C可以产生9摄氏度的温度升高，从而有效地光热杀死癌细胞。MALDI-MS用于证明在Pt NP存在下细胞凋亡相关触发细胞死亡的可能性。结果证实，目前的方法是体内治疗神经癌细胞的有效平台。(C)2013爱思唯尔有限公司保留所有权利。
This study demonstrates the effective synthesis of five different sized/shaped Pt NPs, within a narrow size regime of 1-21 nm using a modified methodology and the toxicity/biocompatibility of Pt NPs on Neuro 2A cancer cells was investigated elaborately by using light microscopic observations, tryphan blue exclusion assay, MIT assay and ICP-MS. The Pt NPs-C with sizes 5-6 nm showed superior non-cytotoxic property compared to the other four Pt NPs. These non-cytotoxic Pt NPs were employed for successful photothermal treatment of Neuro 2A cell lines using near-IR 1064 nm of laser irradiation. The Pt NPs-C could generate a 9 degrees C increase in temperature leading to effective photothermal killing of cancer cells. The MALDI-MS was used to prove the possibility of apoptosis related triggering of cell death in the presence of the Pt NPs. The results confirm that the current approach is an effective platform for in vivo treatment of neuro cancer cells. (C) 2013 Elsevier Ltd. All rights reserved.