Telomerase activity in normal adult brown Norway rat seminal vesicle: Regional distribution and age-dependent changes
Telomerase activity in normal adult brown Norway rat seminal vesicle: Regional distribution and age-dependent changes
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DOI：
10.1210/en.139.3.1075
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发表时间：
1998-03-01
期刊：
影响因子：
4.8
通讯作者：
Brown, TR
中科院分区：
文献类型：
--
作者：
Banerjee, PP;Banerjee, S;Brown, TR
端粒酶活性对于保护细胞免受每轮细胞复制发生的端粒侵蚀是必不可少的，因此似乎在一些但不是所有真核细胞的无限复制潜力中发挥作用。在这方面，一些组织含有具有长增殖寿命并且能够再生或更新组织内的体细胞上皮细胞群的干细胞。由于成年人精囊腺在去势后雄激素替代过程中表现出再生能力，我们假设在成年人精囊腺中存在一个具有再生潜力的细胞库，该细胞表达端粒酶活性。本研究采用一种高灵敏度的基于聚合酶链反应的端粒酶检测方法[端粒重复序列扩增法（TRAP）检测]检测大鼠精囊腺中的端粒酶活性。我们的研究结果表明，端粒酶活性是，确实存在于正常成年大鼠精囊，但在精囊液的存在下，端粒酶活性不能检测。事实上，发现精囊液含有一些对TRAP测定具有抑制作用的因子。此外，我们发现，端粒酶活性在精囊腺的变化与年龄和区域性分布的远端，中间和近端段的管道。这些结果表明，是大鼠前列腺的情况下，一个人口的端粒酶阳性细胞是存在于成年大鼠精囊，从而，这个器官保留整个生命的潜力，再生雄激素替代去势诱导的细胞凋亡。
Telomerase activity is essential for protection of cells against the telomere erosion that occurs with each round of cell replication, and thus appears to play a role in the indefinite replication potential of some, but not all, eukaryotic cells. In this regard, some tissues contain stem cells that have a long proliferative life-span and are capable of regenerating or renewing the somatic epithelial cell population within the tissue. Because the adult seminal vesicle exhibits the ability to regenerate during androgen-replacement after castration, we hypothesized that a pool of cells with regenerating potential is present in the adult seminal vesicle, which expresses telomerase activity. In this study, we used a highly sensitive PCR-based telomerase assay [the telomeric repeat amplification protocol (TRAP) assay] to detect telomerase activity in rat seminal vesicle. Our results show that telomerase activity is, indeed, present in the normal adult rat seminal vesicle, but that, in the presence of seminal vesicle fluid, telomerase activity cannot be detected. In fact, seminal vesicle fluid was found to contain some factor(s) that is inhibitory for the TRAP assay. In addition, we found that telomerase activity in the seminal vesicle changes with age and is regionally distributed within the distal, intermediate, and proximal segments of the duct. These results suggest that as is the case for the rat prostate, a population of telomerase-positive cells is present within the adult rat seminal vesicle, and thereby, this organ retains throughout life the potential to regenerate in response to androgen replacement following castration-induced apoptotic cell death.