Expression of promyelocytic leukemia protein and vascular endothelial growth factor in aqueous humor and vitreous fluid in patients with proliferative diabetic retinopathy
Expression of promyelocytic leukemia protein and vascular endothelial growth factor in aqueous humor and vitreous fluid in patients with proliferative diabetic retinopathy
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DOI：
10.1016/j.diabres.2012.09.020
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发表时间：
2012-11-01
影响因子：
5.1
通讯作者：
Mitamura, Yoshinori
中科院分区：
文献类型：
--
作者：
Katome, Takashi;Namekata, Kazuhiko;Mitamura, Yoshinori
目的：检测增殖性糖尿病视网膜病变(PDR)患者眼组织中早幼粒细胞白血病蛋白(PML)的表达。方法：采用定量RT-PCR方法检测12例PDR患者增殖膜和5例正常对照组增殖膜中PML基因的表达水平。采用ELISA法检测34例PDR患者房水和玻璃体积液中PML和血管内皮生长因子(VEGF)的蛋白表达水平。结果：PDR患者房水和玻璃体积液中PML mRNA表达水平及PML蛋白浓度均显著低于对照组。PDR患者房水和玻璃体积液中PML和VEGF浓度呈显著负相关。结论：PML可作为PDR的诊断标志物和治疗药物。(C)2012爱思唯尔爱尔兰有限公司。保留所有权利。
Aims: To examine the expression of promyelocytic leukemia protein (PML) in the eye of proliferative diabetic retinopathy (PDR) patients.Methods: PML mRNA levels were measured in proliferative membranes from 12 PDR patients and idiopathic epiretinal membranes from 5 control patients by quantitative RT-PCR. Protein levels of PML and vascular endothelial growth factor (VEGF) in aqueous humor and vitreous fluid samples from 34 PDR patients and 38 control patients were determined using ELISA.Results: The PML mRNA expression levels in membrane samples, and the PML protein concentrations in aqueous humor and vitreous fluid samples were significantly lower in PDR patients than control patients. We observed a statistically significant inverse correlation between the concentrations of PML and VEGF in the aqueous humor and vitreous fluid of PDR patients.Conclusion: PML may be a good candidate as a diagnostic marker and a therapeutic agent for PDR. (C) 2012 Elsevier Ireland Ltd. All rights reserved.