INVIVO REGENERATION OF RED-CELL 2,3-DIPHOSPHOGLYCERATE FOLLOWING TRANSFUSION OF DPG-DEPLETED AS-1, AS-3 AND CPDA-1 RED-CELLS
INVIVO REGENERATION OF RED-CELL 2,3-DIPHOSPHOGLYCERATE FOLLOWING TRANSFUSION OF DPG-DEPLETED AS-1, AS-3 AND CPDA-1 RED-CELLS
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DOI：
10.1111/j.1365-2141.1989.tb06286.x
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发表时间：
1989-01-01
影响因子：
6.5
通讯作者：
HOLME, S
中科院分区：
文献类型：
--
作者：
HEATON, A;KEEGAN, T;HOLME, S
将DPG耗尽的0组红细胞输注到A组受体中后，测定2，3-二磷酸甘油酸（DPG）的再生。将来自5个供体的血液在含腺嘌呤的溶液CPDA-1、AS-1或AS-3中于4 ℃储存35天。在7天的时间内，在分离的第0组红细胞中测量输血后红细胞DPG和ATP。这些研究证实了用≥ 100 mg/kg的DPG进行体内快速再生。在7小时内达到最大水平的50%。72小时和7天平均值（±）达到患者输血前DPG水平的95%。SEM）DPG水平相对于患者输血前的DPG平均为84%（± 0.01）。13%）、92%（. ±. 17%）和84%（.+-. CPDA-1、AS-1和AS-3红细胞分别为21%。结果与先前报告的血液在ACD中储存15-20天的结果相当（Valeri和Hirsch，1969; Beutler和Wood，1969）。即时再生率V非常接近一级再生动力学，AS-3红细胞的再生率是CPDA-1红细胞的两倍（P < 0.001）。AS-1红细胞的再生率居中，与CPDA-1和AS-3相比无显著性差异（P > 0.05）。V与输注后5-7 h的ATP水平呈显著正相关（P < 0.05）。输注细胞的ATP再生迅速，在输注后1小时达到高于储存后水平1.2 μ mol/g Hb的平均增加。
Regeneration of 2,3-diphosphoglycerate (DPG) was determined following transfusion of DPG-depleted group 0 red cells into group A recipients. Blood from five donors was stored in the adenine-containing solutions CPDA-1, AS-1 or AS-3 for 35 d at 4.degree.C. Post-transfusion red cell DPG and ATP were measured in separated group 0 red cells over a 7 d period. The studies confirmed rapid in vivo DPG regeneration with .gtoreq. 50% of the maximum level being achieved within 7 h. An average of 95% of the recipient''s pre-transfusion DPG level was achieved by 72 h and by 7 d mean (.+-. SEM) DPG levels relative to recipient''s pre-transfusion DPG averaged 84% (.+-. 13%), 92% (.+-. 17%) and 84% (.+-. 21%) for CPDA-1, AS-1 and AS-3 red cells, respectively. Results were comparable to those previously reported for blood stored in ACD for 15-20 d (Valeri and Hirsch, 1969; Beutler and Wood, 1969). The immediate regeneration rate, V, closely approximated first order regeneration kinetics with AS-3 red cells exhibiting double the rate of CPDA-1 red cells (P < 0.001). AS-1 red cells exhibited an intermediate rate of regeneration which was not significantly different compared to either CPDA-1 or AS-3 (P > 0.05). V exhibited a significant (P < 0.05) positive correlation with ATP levels 5-7 h post-infusion. ATP regeneration of the infused cells was rapid with a mean increase of 1.2 .mu.mol/g Hb above post-storage levels being achieved 1 h following transfusion.