Enrichment of extracellular vesicles with lipid nanoprobe functionalized nanostructured silica.
Enrichment of extracellular vesicles with lipid nanoprobe functionalized nanostructured silica.
复制标题
DOI：
10.1039/c8lc01359d
复制
发表时间：
2019-07
期刊：
影响因子：
6.1
通讯作者：
Y. Wan;Mackenzie Maurer;Hongzhang He;Yiqiu Xia;S. Hao;Wen-Long Zhang;N. Yee;Si-Yang Zheng
中科院分区：
文献类型：
--
作者：
Y. Wan;Mackenzie Maurer;Hongzhang He;Yiqiu Xia;S. Hao;Wen-Long Zhang;N. Yee;Si-Yang Zheng
纳米级细胞外囊泡（nEV）最近在癌症诊断和治疗监测中表现出潜在价值，但转化受到 nEV 分离技术挑战的限制。因此，我们开发了一种一步式 nEV 分离平台，该平台利用 nEV 尺寸匹配的二氧化硅纳米结构和带有集成微流体混合器的表面共轭脂质纳米探针。报道的平台从胰腺癌血浆中的捕获效率为 28.8%，并且可以充分富集 nEV，以便更简单地阳性鉴定胰腺癌患者血浆中 nEV DNA 中的点突变，特别是 KRAS。
Nanoscale extracellular vesicles (nEVs) have recently demonstrated potential value in cancer diagnostics and treatment monitoring, but translation has been limited by technical challenges in nEV isolation. Thus, we have developed a one-step nEV isolation platform that utilizes nEV size-matched silica nanostructures and a surface-conjugated lipid nanoprobe with an integrated microfluidic mixer. The reported platform has 28.8% capture efficiency from pancreatic cancer plasma and can sufficiently enrich nEVs for simpler positive identification of point mutations, particularly KRAS, in nEV DNA from the plasma of pancreatic cancer patients.