Immunoregulatory effects of Glycyrrhizic acid exerts anti-asthmatic effects via modulation of Th1/Th2 cytokines and enhancement of CD4+CD25+Foxp3+ regulatory T cells in ovalbumin-sensitized mice
Immunoregulatory effects of Glycyrrhizic acid exerts anti-asthmatic effects via modulation of Th1/Th2 cytokines and enhancement of CD4+CD25+Foxp3+ regulatory T cells in ovalbumin-sensitized mice
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DOI：
10.1016/j.jep.2013.04.021
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发表时间：
2013-07-30
影响因子：
5.4
通讯作者：
Ma, Shiping
中科院分区：
文献类型：
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作者：
Ma, Chunhua;Ma, Zhanqiang;Ma, Shiping
民族药理学相关性：甘草酸（GA）是甘草的主要活性成分，具有多种治疗作用，本研究旨在探讨甘草酸对哮喘的免疫调节作用及其机制。将60只小鼠随机分为6个实验组：对照组、模型组、地塞米松（2 mg/kg）组和GA（10 mg/kg、20 mg/kg、40 mg/kg）组。采用强迫振荡法测定气道阻力（Raw），HE染色观察气道组织学改变，酶联免疫吸附试验（ELISA）检测Th 1/Th 2和Th 17细胞因子，流式细胞术（FCM）检测CD 4（+）CD 25（+）Foxp 3（+）调节性T细胞（TcR）。结果：与模型组相比，GA可抑制OVA诱导的Raw和嗜酸性粒细胞计数的增加;支气管肺泡灌洗液中IL-4、IL-5、IL-13水平恢复正常，IFN-γ水平升高;组织学研究表明，与模型组相比，GA显著抑制OVA诱导的肺组织和气道组织嗜酸性粒细胞增多。结论：GA能有效地改善哮喘的进展，可作为治疗过敏性哮喘的一种有效方法。(C)2013爱思唯尔爱尔兰有限公司版权所有。
Ethnopharmacological relevance: Glycyrrhizic acid (GA) is the main bioactive ingredient of licorice (Glycyrrhiza glabra), and has been found to be associated with multiple therapeutic properties.Aim of the study: In this study, we investigated immunoregulatory effects of glycyrrhizic acid on anti-asthmatic effects and underlying mechanisms.Materials and methods: Asthma model was established by ovalbumin-induced. A total of 60 mice were randomly assigned to six experimental groups: control, model, dexamethasone (2 mg/kg) and GA (10 mg/kg, 20 mg/kg, 40 mg/kg). Airway resistance (Raw) were measured by the forced oscillation technique, histological studies were evaluated by The hematoxylin and eosin (HE) staining, Th1/Th2 and Th17 cytokines were evaluated by enzyme-linked immunosorbent assay (ELISA), and CD4(+)CD25(+)Foxp3(+) regulatory T cells (Tregs) was evaluated by Flow Cytometry (FCM), the forkhead/winged helix transcription factor (Foxp3) was evaluated by western blotting.Results: Our study demonstrated that, compared with model group, GA inhibited OVA-induced increases in Raw and eosinophil count; interleukin (IL)-4, IL-5, IL-13 levels were recovered in bronchoalveolar lavage fluid compared; increased IFN-gamma level in bronchoalveolar lavage fluid; histological studies demonstrated that GA substantially inhibited OVA-induced eosinophilia in lung tissue and airway tissue compared with model group. Flow cytometry studies demonstrated that GA substantially enhanced Tregs compared with model group.Conclusion: These findings suggest that GA may effectively ameliorate the progression of asthma and could be used as a therapy for patients with allergic asthma. (C) 2013 Elsevier Ireland Ltd. All rights reserved.