CORRELATION OF CHROMOSOMAL-ABERRATIONS IN A MYELOMA CELL-LINE WITH TUMORIGENICITY IN NUDE-MICE
CORRELATION OF CHROMOSOMAL-ABERRATIONS IN A MYELOMA CELL-LINE WITH TUMORIGENICITY IN NUDE-MICE
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DOI：
10.1007/bf00320467
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发表时间：
1981-01-01
期刊：
影响因子：
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通讯作者：
DIEHL, V
中科院分区：
文献类型：
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作者：
KIRCHNER, HH;FONATSCH, C;DIEHL, V
人类骨髓瘤细胞系 (L363) 表现出多种恶性肿瘤标记，包括 14q+ 染色体标记，在裸鼠中不具有致瘤性。体外长期培养产生了与原始品系具有轻微、明确的染色体差异的新亚克隆。某些亚克隆在体外具有生长优势，移植到裸鼠后会产生致瘤性。在两个实验系统中发现，导致长臂材料剂量增加 3 倍的 1 号染色体星座的变化与增殖潜力的增加之间存在很强的相关性。
A human myeloma cell line (L363) exhibiting several markers of malignancy including a 14q+ chromosome marker was not tumorigenic in nude mice. Long-term cultivation in vitro resulted in new subclones with slight, well-defined chromosomal differences to the original line. Certain subclones had a growth advantage in vitro and became tumorigenic after transplantation in nude mice. A strong correlation between changes in the chromosome 1 constellation resulting in the 3-fold dose of long arm-material and the increased proliferative potential in both experimental systems was found.